Genotypes of Listeria monocytogenes strains isolated from 2000 to 2002 in Poland.

Pulsed field gel electrophoresis (PFGE), multiplex PCR and multilocus sequence typing (MLST) methods were used for genotyping study of seventy-three L. monocytogenes isolates collected in Poland between 2000 and 2002 from human, food, environment and a diseased goat. The multiplex PCR, which is an alternative method to classical serotyping, divided the isolates into four PCR groups, IIa (42.

Antimicrobial susceptibilities of Listeria monocytogenes strains isolated from 2000 to 2002 in Poland.

Minimal inhibitory concentration (MIC) of ampicillin, penicillin, gentamicin, erythromycin, clarithromycin, sulfisoxazole and trimethoprim for 73 Listeria monocytogenes isolates from clinical material samples, food and environment was determined using broth microdilution method. With exception of the sulfonamide all antibacterial agents were active against all tested strains. Resistance to sulfisoxazole (MIC > or = 512 microg/ml) was detected in case of 30.

[Application of PCR to Haemophilus influenzae typing. I. PCR standardization for bexA gene fragment detection and for detection of DNA fragment specific for H.influenzae type B].

PCR standardization was performed in order to detect a fragment of bexA gene, which is presented in all capsulate H. influenzae isolates, and a DNA fragment specific for H. influenzae type b.

[Phage types and plasmid profiles of plasmid DNA strains of Salmonella enterica subsp. enterica ser. Enteritidis (S. Enteritidis) isolated from food poisoning outbreaks in 2001].

Salmonella Enteritidis strains are the most often isolated Salmonella serovar in Poland. In the present study, phage typing, antibiotic resistance testing and plasmid profile analysis, have been applied to characterise 41 Polish S. Enteritidis isolates originated from human cases of salmonellosis and from other sources.

Virulence properties of Escherichia coli faecal strains isolated in Poland from healthy children and strains belonging to serogroups O18, O26, O44, O86, O126 and O127 isolated from children with diarrhoea.

Four hundred and twenty-seven Escherichia coli isolates from 427 cases of infantile diarrhoea in Poland, belonging to serogroups O18, O26, O44, O86, O126 and O127 and 150 E. coli isolates from 52 healthy children were examined for selected virulence properties. The presence of the plasmid pAA, a plasmid encoding enterohaemolysin, the genes encoding intimin (eae), bundle-forming pili (bfp), Shiga toxins I and II (stxI, stxII) and cytotoxic necrotising factor types 1 and 2 (cnfl, cnf2) was investigated by PCR.