Publications by authors named "Jaroslava Geleticova"
We examined the inhibitory effects of three flavonolignans and their dehydro- derivatives, taxifolin and quercetin on the activity of the Na(+)/K(+)-ATPase (NKA). The flavonolignans silychristin, dehydrosilychristin and dehydrosilydianin inhibited NKA with IC50 of 110 ± 40 μM, 38 ± 8 μM, and 36 ± 14 μM, respectively. Using the methods of molecular modeling, we identified several possible binding sites for these species on NKA and proposed the possible mechanisms of inhibition.
View Article and Find Full Text PDFArticle Synopsis
- Membrane proteins are challenging to study due to their poor solubility and instability outside lipid environments, prompting research into their incorporation into model lipid systems like liposomes.
- A novel electrochemical method has been introduced that allows for the analysis of transmembrane proteins embedded in liposomes by measuring the electrical signals of these proteins on electrode surfaces.
- This label-free approach provides a way to study these proteins at low concentrations and could lead to advancements in electrochemical sensors and microarray technologies for proteins that are difficult to dissolve in water.
Na(+)/K(+)-ATPase inhibition by cisplatin and consequences for cisplatin nephrotoxicity.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub
June 2014
Aims: Cisplatin is a widely used chemotherapeutic. However, it is associated with numerous adverse effects. The aim of our study was examination of cisplatin interaction with Na(+)/K(+)-ATPase (NKA, the sodium pump).
View Article and Find Full Text PDF