Development of a graphene field effect transistor-based immersible biosensor for immunodetection of the birch pollen allergen Bet v 1 in air samples.

Pollen traps, the current gold standard to determine pollen load and thereby the allergy season, are not sufficient to determine the allergenic risk. Therefore, the establishment of highly sensitive assays for allergen measurement is of highest interest. Herein, a graphene field-effect transistor (GFET) was constructed on an interdigitated electrodes chip to develop an immersible biosensor, which was used to detect the major birch pollen allergen Bet v 1.

Relationship between potentially toxic elements and macrophyte communities in the Sava river.

Freshwater ecosystems are at significant risk of contamination by potentially toxic elements (PTEs) due to their high inherent toxicity, their persistence in the environment and their tendency to bioaccumulate in sediments and living organisms. We investigated aquatic macrophyte communities and the concentrations of As, Cu, Cd, Cr, Pb, Zn, Ni and Fe in water and sediment samples to identify a pollution pattern along the Sava River and to investigate the potential impact of these PTEs on the diversity and structure of macrophyte communities. The study, which covered 945 km of the Sava River, showed a downstream increase in sediment concentrations of the analyzed elements.

Phytoremediation potential of invasive plant species for potentially toxic elements along the Sava River upstream.

Invasive plant species (IPS) have many characteristics that are necessary for successful phytoremediation and the accumulation of large amounts of potentially toxic elements (PTEs). The most common IPS from the source of the Sava River are Re and . Considering that the riparian soils of the Sava River are classified as moderately polluted, this study investigated their enrichment with PTEs (As, B, Cd, Cr, Cu, Ni, Pb, Zn) and the potential for accumulation of these elements in roots and leaves of the most common IPS.

Biosensors for Cancer Biomarkers Based on Mesoporous Silica Nanoparticles.

Mesoporous silica nanoparticles (MSNs) exhibit highly beneficial characteristics for devising efficient biosensors for different analytes. Their unique properties, such as capabilities for stable covalent binding to recognition groups (e.g.

Comparative Study of Field-Effect Transistors Based on Graphene Oxide and CVD Graphene in Highly Sensitive NT-proBNP Aptasensors.

Graphene-based materials are actively being investigated as sensing elements for the detection of different analytes. Both graphene grown by chemical vapor deposition (CVD) and graphene oxide (GO) produced by the modified Hummers' method are actively used in the development of biosensors. The production costs of CVD graphene- and GO-based sensors are similar; however, the question remains regarding the most efficient graphene-based material for the construction of point-of-care diagnostic devices.

Direct electrochemical reduction of graphene oxide thin film for aptamer-based selective and highly sensitive detection of matrix metalloproteinase 2.

Simple and low-cost biosensing solutions are suitable for point-of-care applications aiming to overcome the gap between scientific concepts and technological production. To compete with sensitivity and selectivity of golden standards, such as liquid chromatography, the functionalization of biosensors is continuously optimized to enhance the signal and improve their performance, often leading to complex chemical assay development. In this research, the efforts are made on optimizing the methodology for electrochemical reduction of graphene oxide to produce thin film-modified gold electrodes.

Green solution to riparian pollution: L. potential for phytoremediation and bioindication of PTEs along the Sava river.

This study addresses the potential of L. for bioindication and phytoremediation of the contaminated lower part of the Sava River. The main objectives are to assess soil contamination with potentially toxic elements (PTEs: As, B, Cd, Cr, Cu, Li, Ni, Pb, Sr, and Zn), evaluate their availability, and assess the phytoremediation and bioindication potential of .

Ecophysiological response of L. to multiple stress factors during the revitalisation of coal fly ash lagoons at different stages of weathering.

The enormous quantities of fly ash (FA) produced by thermal power plants is a global problem and safe, sustainable approaches to reduce the amount and its toxic effects are still being sought. Vegetation cover comprising long-living species can help reduce FA dump-related environmental health issues. However, the synergistic effect of multiple abiotic factors, like drought, low organic matter content, a deficit of essential nutrients, alkaline pH, and phytotoxicity due to high potentially toxic element (PTE) and soluble salt content, limits the number of species that can grow under such stressful conditions.

Ethnobotanical and ethnomedicinal research into medicinal plants in the Mt Stara Planina region (south-eastern Serbia, Western Balkans).

Background: Ethnobotanical research in Southeast Europe-one of the most important European hotspots for biocultural diversity-is significant for the acquisition of Traditional Ecological Knowledge related to plants as well as for encouraging the development of local environments. The current ethnobotanical research was conducted in the region of Mt Stara Planina (south-eastern Serbia), which is characterised by rich phytodiversity with a large number of endemic and relict plant species. The aim of the study was to document the diversity of uses of medicinal plants and of traditional knowledge on their therapeutic uses.

Lithium and strontium accumulation in native and invasive plants of the Sava River: Implications for bioindication and phytoremediation.

The objective of this study was to investigate the potential of native and invasive plant species for the uptake and accumulation of lithium (Li) and strontium (Sr) along the Sava River, focusing on their bioindication and phytoremediation capabilities. Sampling was carried out in riparian zones exposed to different pollution sources in Slovenia, Croatia, and Serbia. Plant samples of native (Salix alba, Populus alba, Populus nigra, Ulmus glabra, Juglans regia) and invasive (Amorpha fruticosa, Reynoutria japonica, Solidago canadensis, Impatiens glandulifera) species were collected.

Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor.

In this work, we report a novel method for the label-free detection of cyanotoxin molecules based on a direct assay utilizing a graphene-modified surface plasmon resonance (SPR) aptasensor. Molecular dynamic simulation of the aptamer's interaction with cylindrospermopsin (CYN) reveals the strongest binding sites between C18-C26 pairs. To modify the SPR sensor, the wet transfer method of CVD monolayer graphene was used.

The Measurement of Contact Angle, pH, and Conductivity of Artificial Saliva and Mouthwashes on Enamel, Glass-Ionomer, and Composite Dental Materials.

The aim of this study was to tackle the topic of appropriate recommendations for artificial-saliva and mouthwash usage. The contact angle, pH, and conductivity of two artificial saliva solutions, four mouthwashes, and their mixtures on enamel, glass-ionomer, and composite dental materials were measured. The measurements were conducted with a MATLAB algorithm to minimize human error.

The Phytoremediation Potential and Physiological Adaptive Response of Pall. Ex M. Bieb. during the Restoration of Chronosequence Fly Ash Deposits.

The challenging process of identifying and selecting plant species suited to the phytoremediation of fly ash (FA) dumps involves studying their functional properties and physiological response to a deficit of essential elements and toxicity from heavy metal(loid)-induced oxidative stress. We hypothesised that has high potential to be used for the phytoremediation of FA deposit sites thanks to its secretion strategy and antioxidative system. In this study, this hypothesis was examined by determining the bioconcentration and translocation factors for As, B, Cr, Cu, Mn, Ni, Se and Zn at the FA disposal lagoons at the 'Nikola Tesla A' thermal power plant in Obrenovac, Serbia, three (lagoon L1) and eleven (lagoon L2) years after the phytoremediation process had begun, and by measuring parameters of photosynthetic efficiency and chlorophyll concentration, non-enzymatic antioxidant defence (carotenoids, anthocyanins and phenolics), oxidative stress (concentration of malondialdehyde-MDA) and total antioxidant capacity to neutralise DPPH free radical activity.

Hieracium waldsteinii (Asteraceae) and Onosma stellulata (Boraginaceae) as a Source of Antioxidant and Antimicrobial Agents.

The current study aimed to phytochemically characterize (including a detailed phenolic profile) two endemic Balkan's species (Hieracium waldsteinii and Onosma stellulata) and determine their possible application as a source of natural antioxidant and antimicrobial agents. The main phenolic compound in both species (in all examined parts) was chlorogenic acid. Eriodictyol, genistein and naringenin were quantified only in H.

An Assessment of the Phytoremediation Potential of Planted and Spontaneously Colonized Woody Plant Species on Chronosequence Fly Ash Disposal Sites in Serbia-Case Study.

In this study, the potential of planted ( Pall. ex M.Bieb.

Real-time detection of ochratoxin A in wine through insight of aptamer conformation in conjunction with graphene field-effect transistor.

Mycotoxins comprise a frequent type of toxins present in food and feed. The problem of mycotoxin contamination has been recently aggravated due to the increased complexity of the farm-to-fork chains, resulting in negative effects on human and animal health and, consequently, economics. The easy-to-use, on-site, on-demand, and rapid monitoring of mycotoxins in food/feed is highly desired.

Advances in Nanomaterials-Based Electrochemical Biosensors for Foodborne Pathogen Detection.

Electrochemical biosensors utilizing nanomaterials have received widespread attention in pathogen detection and monitoring. Here, the potential of different nanomaterials and electrochemical technologies is reviewed for the development of novel diagnostic devices for the detection of foodborne pathogens and their biomarkers. The overview covers basic electrochemical methods and means for electrode functionalization, utilization of nanomaterials that include quantum dots, gold, silver and magnetic nanoparticles, carbon nanomaterials (carbon and graphene quantum dots, carbon nanotubes, graphene and reduced graphene oxide, graphene nanoplatelets, laser-induced graphene), metal oxides (nanoparticles, 2D and 3D nanostructures) and other 2D nanomaterials.

Phytoremediation Potential, Photosynthetic and Antioxidant Response to Arsenic-Induced Stress of L. Sown on Fly Ash Deposits.

Arsenic (As) from coal fly ash can be released into soil/groundwater, presenting a global threat to the environment and human health. To overcome this environmental problem, phytoremediation represents an urgent need, providing 'green' cleanup of contaminated lands. The present study focused on As concentrations in fly ash and plants, evaluation of phytoremediation potential of sown on fly ash deposits together with its photosynthetic activity, and oxidative and antioxidative response to As stress.

Evaluation of Salix alba, Juglans regia and Populus nigra as biomonitors of PTEs in the riparian soils of the Sava River.

A large number of human activities result in the release of potentially toxic elements (PTEs) into the environment, which could lead to the degradation of riparian areas. This study aimed to evaluate the potential of Salix alba, Juglans regia and Populus nigra for the biomonitoring of PTEs in the riparian soils of the Sava River. Levels of seven PTEs (As, Cd, Cr, Cu, Ni, Pb and Zn) were measured in the soils, roots and leaves of plants at selected sampling sites and evaluated according to bioaccumulation and translocation factors.

The potential of elm trees (Ulmus glabra Huds.) for the phytostabilisation of potentially toxic elements in the riparian zone of the Sava River.

The use of trees to immobilise potentially toxic elements (PTEs) is a low-cost and effective method of soil remediation. Thus, the objective of this study was to assess the content of total and bioavailable As, Cd, Cr, Cu, Ni, Pb and Zn in soil samples, as well as their levels in the roots and leaves of elm (Ulmus glabra Huds.) in order to evaluate its potential for the phytostabilisation of PTEs in the riparian zone of the Sava River.

Is Test Standardization Important when Arm and Leg Muscle Mechanical Properties are Assessed through the Force-Velocity Relationship?

The force-velocity (F-V) relationship observed in multi-joint tasks proved to be strong and approximately linear. Recent studies showed that mechanical properties of muscles: force (F), velocity (V) and power (P) could be assessed through the F-V relationship although the testing methods have not been standardized. The aim of the present study was to evaluate and compare F-V relationships assessed from two tests performed on a modified Smith machine that standardizes kinematics of the movement pattern.

Reliability of force-velocity parameters obtained from linear and curvilinear regressions for the bench press and squat exercises.

This study aimed to compare the goodness of fit and the reliability of different regression models for fitting the force-velocity relationship (FV) of bench press (BP) and squat (SQ). Additionally, the reliability of the position on FV of the velocity (V) and the force performed with the 1RM (F) was explored. Nine rugby players and 12 judokas participated in this study.

Self-Preferred Initial Position Could Be a Viable Alternative to the Standard Squat Jump Testing Procedure.

Petronijevic, MS, Garcia Ramos, A, Mirkov, DM, Jaric, S, Valdevit, Z, and Knezevic, OM. Self-preferred initial position could be a viable alternative to the standard squat jump testing procedure. J Strength Cond Res 32(11): 3267-3275, 2018-The purpose of this study was to compare both the magnitude and reliability of different variables (knee angle, squat depth, jump height [Hmax], maximum force [Fmax], and maximum power [Pmax]) between the standardized squat jump (SJ) and the SJ performed from the self-preferred position.

Optimisation of applied loads when using the two-point method for assessing the force-velocity relationship during vertical jumps.

This study aimed to compare the reliability and validity of the force-velocity (F-V) relationship parameters obtained from two-point methods differing in the distance between experimental points, and to evaluate the acute change in unloaded jump height after a vertical jump testing procedure based on multiple loads. Totally, 18 men randomly performed two sessions of the squat jump (SJ) and two sessions of the countermovement jump (CMJ) exercises against five external loads (17, 30, 45, 60 and 75 kg). The unloaded jump height was evaluated before and after each testing procedure.

Effects of Reduced Effort on Mechanical Output Obtained From Maximum Vertical Jumps.

The aim of this study was to evaluate the effect of reduced effort on maximum countermovement jumps. Groups of unskilled and skilled jumpers performed countermovement jumps without an arm swing at 100% and 50% effort. The results revealed markedly reduced jump height and work performed at 50% effort, although the maximum force and power output remained virtually unchanged.