Characterization of the Polyphenolic Profile in Tomato ( P. Mill) Peel and Seeds by LC-HRMS/MS.

Peel and seeds are the main byproducts from tomato ( P. Mill) processing with high concentrations of polyphenols that have been underexploited. Herein, polyphenolic profiles in tomato peel and seeds were elucidated by untargeted liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) with an LTQ Orbitrap analyzer.

A paper-based analytical device with in-situ Carrez pretreatment for the determination of total polyphenolic content and antioxidant capacity.

A µPAD to determine the total polyphenol content (TPC) by Folin-Ciocalteau (FC) method, and antioxidant capacity by ABTS method, coupling an in-situ pretreatment zone with Carrez reagents is proposed. Conditions of FC, KSO, ABTS reagents concentrations, pH, and incubation were established using 2 factorial designs. The proposed µPAD showed linearities upper than 0.