Transmitted Antiretroviral Drug Resistance to Integrase Strand Transfer Inhibitors Class in São Paulo Metropolitan Area, Brazil.

A newer integrase strand transfer inhibitor (INSTI) cabotegravir was recently approved for both therapy and prophylaxis and can play an essential role in the fight against AIDS. It shares similar resistance profile to dolutegravir, the cornerstone of Brazilian antiretroviral (ARV) treatment, with about 600 thousand people living with HIV in Brazil currently on regimens that contain this INSTI. Health services in the São Paulo metropolitan area are responsible for a large proportion of ARV dispensation in the country.

Estrogen Deficiency Impairs Osseointegration in Hypertensive Rats Even Treated with Alendronate Coated on the Implant Surface.

Hypertension and estrogen deficiency can affect bone metabolism and therefore increase the risk of osseointegration. Antihypertensive drugs such as losartan not only control blood pressure but also enhance bone healing. In addition, alendronate sodium is widely used to treat postmenopausal osteoporosis.

Adolescent mothers, self-care and childcare: content validation of an Event History Calendar.

Objective: To validate the content of the tool Event History Calendar Adolescent Mother: strengthening self-care and child care.

Method: Methodological study using the Delphi technique, conducted in two rounds, involving 37 nursing specialists. In data collection, from December/2019 to August/2020, a semi-structured questionnaire composed of 47 items related to the two dimensions of the tool: Self-care and Child Care was used.

Assessing the Fate of Superparamagnetic Iron Oxide Nanoparticles Carrying Usnic Acid as Chemical Cargo on the Soil Microbial Community.

In the present study we evaluate the effect of superparamagnetic iron oxide nanoparticles (SPIONs) carrying usnic acid (UA) as chemical cargo on the soil microbial community in a dystrophic red latosol (oxysol). Herein, 500 ppm UA or SPIONs-framework carrying UA were diluted in sterile ultrapure deionized water and applied by hand sprayer on the top of the soil. The experiment was conducted in a growth chamber at 25 °C, with a relative humidity of 80% and a 16 h/8 h light-dark cycle (600 lx light intensity) for 30 days.

Use of Sanger protocols to identify variants of concern, key mutations and track evolution of SARS-CoV-2.

Vaccination and the emergence of SARS-CoV-2 variants mark the second year of the pandemic. Variants have amino acid mutations at the spike region, a viral protein central in the understanding of COVID-19 pathogenesis and vaccine response. Variants may dominate local epidemics, as Gamma (P.

Effects of substrates containing different concentrations of sewage sludge on physiological parameters and quality of Alibertia edulis (Rubiaceae) seedlings.

Sewage sludge (SS), a solid residue of effluent treatment, is rich in organic matter and nutrients, while also containing heavy metals and other potential contaminants. The feasibility of employing SS as a substrate component for seedling production depends on its composition and dose, as well as on the tolerance limit of individual plant species. To expand the knowledge base on the use of SS in the production of plants native to the Brazilian Cerrado biome, we evaluated the physiological responses and quality of Alibertia edulis seedlings grown under distinct SS concentrations.

Strengthening Self-Care and Child Care of Adolescent Mothers Through an Event History Calendar.

Purpose: To identify events and experiences of adolescent mothers relevant to their own care and the care of their children so as to support the elaboration of a future Event History Calendar (EHC) tool.

Design And Methods: Qualitative study was conducted based on the Grounded Theory, from the constructivist perspective. Data were collected through in-depth interviews with 11 Brazilian adolescent mothers.

Physiological mechanisms and phytoremediation potential of the macrophyte Salvinia biloba towards a commercial formulation and an analytical standard of glyphosate.

Glyphosate (Gly) is the most widely used herbicide in the world and has broad-spectrum and non-selective activity. Its indiscriminate use hence risks contamination of water bodies and can affect living organisms, especially sensitive or resistant non-target plants. Despite this, studies on physiological mechanisms and Gly remediation in Neotropical aquatic plants remain limited.

Maternal-child nursing care for adolescent mothers: health education.

Objectives: to develop an educational intervention through a game that addresses aspects related to adolescent motherhood and child care.

Methods: this is an action research based on diagnosis, intervention and apprehension stages.

Results: the situational diagnosis was based on a literature review on adolescent motherhood and child care.

Nurse to adolescent health communication process: approach to Event History Calendar.

Objectives: comprehend the Event History Calendar components that are relevant for the nurse to adolescent communicative process, in the context of Primary Health Care.

Methods: reflective study, based on the Event History Calendar approach, in the relational, communicative, and educational dimensions.

Results: best practices for adolescent health promotion are vital and constitute a challenge to nurses.

Purification of IgG against ribonucleoprotein by a homemade immunoaffinity chromatography column for rabies diagnosis.

Polyclonal or monoclonal antibodies against rabies virus ribonucleoprotein (RNP) conjugated to fluorescein isothiocyanate (FITC) have been employed for Rabies virus (RABV) antigen detection by the direct fluorescent antibody test (DFA). To date, these biomolecules have been purified by traditional methods such as precipitation by ammonium sulfate or ion exchange chromatography followed by ammonium sulfate precipitation, which allows only for partial detection of the protein of interest. In this study, we aimed to purify anti-RNP polyclonal horse IgG antibodies by cation-exchange chromatography in combination with a homemade immunoaffinity chromatography on RNP immobilized (RNP-IAC).

[Child care and health rights: perspectives of adolescent mothers].

Objective: To analyze child health care and the defense of their rights from the perspective of adolescent mothers.

Methods: An exploratory study with qualitative thematic analysis of data, based on conceptual aspects of care and the right to health, from semi-structured interviews with 20 adolescent mothers ascribed by Family Health teams.

Results: Maternal reports indicate that child health care requires responsibility and protection, with health practices that promote child advocacy.