Understanding microbiome dynamics and functional responses during acidogenic fermentation of sucrose and sugarcane vinasse through metatranscriptomic analysis.

Improving anaerobic digestion of sugarcane vinasse - a high-strength wastewater from ethanol distillation - is a subject of great interest, in view of the reduction of the pollutants and recovery of methane and valuable metabolites as byproducts. Through metatranscriptomic analysis, this study evaluated the active microbiome and metabolic pathways in a continuous acidogenic reactor: Stage 1S (control): 100% sucrose-based substrate (SBS); Stage 2SV (acclimation): 50% SBS and 50% vinasse; Stage 3V: 100% vinasse. Metatranscriptome obtained from each Stage was subjected to taxonomic and functional annotations.

Hydrogen and organic acid production from dark fermentation of cheese whey without buffers under mesophilic condition.

BioH production from cheese whey (CW) was evaluated in two acidogenic reactors, UASB and structured fixed-bed (FB), without pH adjustment, under mesophilic conditions, and OLR of 25-90 g COD/L.d. Stage 1 was conducted as a control experiment using sucrose.