Electroporation as a Solvent-Free Green Technique for Non-Destructive Extraction of Proteins and Lipids From .

Proteins extracted from microalgae for food, personal care products and cosmetics must be of high purity, requiring solvent-free extraction techniques despite their generally considerably lower protein yield and higher energy consumption. Here, three such approaches for green extraction of proteins from were evaluated: ultrasound, freeze-thawing, and electroporation; chemical lysis was used as positive control (maximal achievable extraction), and no extraction treatment as negative control. Compared to chemical lysis, electroporation yielded the highest fraction of extracted protein mass in the supernatant (≤27%), ultrasound ≤24%, and freeze-thawing ≤15%.

Scale-up research in a dual fluidized bed gasification process.

A successful co-gasification of plastics and biomass was achieved on the 100 kW dual fluidized bed (DFB) gasification pilot plant. The results of a pilot plant experiment were used as a sound basis for scale-up prediction to 750 kW semi-industrial DFB plant. By an eightfold increase of mass and heat flows a rather simplified co-gasification process was predicted.

Potential Solutions for CO2-capturing Technologies in the Slovenian Context.

Human activities have caused an enormous rise of the CO2 concentration in the Earth's atmosphere over the past 200 years. In order to alleviate this problem, the threats to and the concerns of the international community need to be converted into economic opportunities for national economies, which shall develop and utilize technological opportunities rather than simply accepting international obligations to reduce CO2 emissions. In the article we analyze technological possibilities in the Slovenian context as possible opportunities for promoting sustainable development based on regional, renewable resources.

Growth, lipid extraction and thermal degradation of the microalga Chlorella vulgaris.

The microalga Chlorella vulgaris was cultured in a combined medium obtained by mixing standard Jaworski medium with a solution from the modified Solvay process that contained only NaHCO(3) and NH(4)Cl. Cell number, pH and nitrogen content were monitored throughout growth. Lipids were extracted from lyophilised biomass using CHCl(3)-MeOH.

Impact of the buildings areas on the fire incidence.

A survey of statistical studies shows that probability of fires is expressed by the equation P(A) = KAα, where A = total floor area of the building and K and  are constants for an individual group, or risk category. This equation, which is based on the statistical data on fires in Great Britain, does not include the impact factors such as the number of employees and the activities carried out in these buildings. In order to find out possible correlations between the activities carried out in buildings, the characteristics of buildings and number of fires, we used a random sample which included 134 buildings as industrial objects, hotels, restaurants, warehouses and shopping malls.