RIOT-Rapid Immunoglobulin Overview Tool-annotation of nucleotide and amino acid immunoglobulin sequences using an open germline database.

Antibodies are a cornerstone of the immune system, playing a pivotal role in identifying and neutralizing infections caused by bacteria, viruses, and other pathogens. Understanding their structure, and function, can provide insights into both the body's natural defenses and the principles behind many therapeutic interventions, including vaccines and antibody-based drugs. The analysis and annotation of antibody sequences, including the identification of variable, diversity, joining, and constant genes, as well as the delineation of framework regions and complementarity-determining regions, is essential for understanding their structure and function.

Benchmarking antibody clustering methods using sequence, structural, and machine learning similarity measures for antibody discovery applications.

Antibodies are proteins produced by our immune system that have been harnessed as biotherapeutics. The discovery of antibody-based therapeutics relies on analyzing large volumes of diverse sequences coming from phage display or animal immunizations. Identification of suitable therapeutic candidates is achieved by grouping the sequences by their similarity and subsequent selection of a diverse set of antibodies for further tests.

nanoBERT: a deep learning model for gene agnostic navigation of the nanobody mutational space.

Motivation: Nanobodies are a subclass of immunoglobulins, whose binding site consists of only one peptide chain, bestowing favorable biophysical properties. Recently, the first nanobody therapy was approved, paving the way for further clinical applications of this antibody format. Further development of nanobody-based therapeutics could be streamlined by computational methods.

Spectrum of complaints: practices of complaining in therapeutic conversations as a window to spouses' personalities and couples' relationships.

Introduction: Complaining is a frequent phenomenon in human interactions and it frequently happens during couple counseling. A conversation between a therapist and spouses that requires them to talk about problems inevitably leads to complaining (especially during the first meeting). The institutional context and the presence of an impartial therapist shape the complaining sequences.

How therapists respond to "uneven" alliances in couple and family therapy: A conversation-analytic study.

This article examines how family and couple therapists respond to uneven alliances with their clients at the micro-level of therapeutic exchanges in the context of Interpersonal Process Recall (IPR) interviews. We operationalize uneven alliance with the interactional concept of asymmetry of affiliation. To this end, first, using conversation analysis (CA), we identify episodes of asymmetry of affiliation in the moment-by-moment conversation between the therapist and the client in therapy consultation.

Structural modeling of antibody variable regions using deep learning-progress and perspectives on drug discovery.

AlphaFold2 has hallmarked a generational improvement in protein structure prediction. In particular, advances in antibody structure prediction have provided a highly translatable impact on drug discovery. Though AlphaFold2 laid the groundwork for all proteins, antibody-specific applications require adjustments tailored to these molecules, which has resulted in a handful of deep learning antibody structure predictors.

Time in Grief: How do Bereaved Parents Mentalize It?

In this multimethod study, we examine bereaved parents' capacity for mentalizing the temporal dimension of their grief. The theoretical assumptions of our study draw on the clinical and anthropological perspectives on the passage of time in grief. Parents' mentalization of their experience of grief was measured both in the attachment context, using the Adult Attachment Interview (AAI) and using the narrative Child Loss Interview (CLI).

Parental experiences of the liminal period of a child's fatal illness.

The article offers a description of parents' experiences of their child's ultimately fatal illness as it unfolds over the successive stages of medical treatment, in the context of the liminality theory. The parents ( = 23) were interviewed 1-4 years after their child's death. The research method involved conducting narrative interviews with parents in order to obtain a spontaneous narration of the child's illness as it unfolded.

Practices of Claiming Control and Independence in Couple Therapy With Narcissism.

Four couple therapy first consultations involving clients with diagnosed narcissistic problems were examined. A sociologically enriched and broadened concept of narcissistic disorder was worked out based on Goffman's micro-sociology of the self. Conversation analytic methods were used to study in detail episodes in which clients resist to answer a therapist's question, block or dominate the development of the conversation's topic, or conspicuously display their interactional independence.

How couple therapists manage asymmetries of interaction in first consultations.

The study demonstrates how asymmetries in therapists' affiliations with spouses emerge and are addressed in couple therapy. A total of 4 video-recorded couple therapy first sessions were subjected to conversation analysis. The moment-by moment interactions that contribute to one sided affiliation, as well as the therapists' ways of managing such asymmetry, are described in detail.

Does the family "need" depression? A pilot study of family consultations.

Objectives: The aim of the presented study was to analyse associations between drug-resistant depression and the way the illness is described by patients and members of their families. In particular, a hypothesis to be verified was that being ill may be a factor stabilising the family system, and consequently treatment of this kind of depression may encounter additional difficulties and enforce "drug-resistance" by "sustaining depression" by the family.

Methods: The study included 20 patients and their families.

The Rites of Passage Framework as a Matrix of Transgression Processes in the Life Course.

This work shows the contribution of concept of rites of passage and theory of liminality to the understanding of transformations in the course of a person's life. The structural-functional analysis of empirical studies of physical changes, changing roles in society, and key changes in the area of mental and physical health conducted from the perspective of these theories has allowed to identify the three fundamental processes that govern the attainment of transformation and transgression into a new phase of life. The aim of this paper is to set out the processes identified in the course of functional-structural analysis of chosen studies and they comprise: (1) preservation of the sequence of the life course; (2) liminality: deconstruction, integration, and transformation; and (3) performativity.

Characteristics of mentalization patterns in parents of children with difficulties in realizing developmental objectives of the latency stage - qualitative analysis results.

Objectives: The aim of the study was to answer the question about the ability to mentalize emotional states in parents of children with difficulties in realizing developmental objectives of the latency stage. The research was exploratory in nature; the aim was formulated on the basis of notions from the field literature indicating a correlation between the attachment style and the ability to mentalize in the parents and psychosocial functioning of their children.

Methods: The structured Adult Attachment Interview (AAI) was used as the main tool for the research.

[The influence of unreconciled grief in the family on the functioning and development of a child].

The aim of the work is to present the influence of unreconciled grief in a family for functioning and growth of a child. The paper is based on some examples of clinical work from the field of family therapy, where developmental problems of children followed their carer's inability to cope with death and bereavement. Presented cases from family therapy serve as examples of possible therapeutic interventions in such situations.

Maternal bond with cardiosurgically treated infant. Qualitative analysis of mothers' narratives.

The aim of this work is to describe the experience of being a mother by women who together with their children stay on the ward after cardiac surgical correction of congenital heart defects. The research material consisted of the narratives of mothers whose children were born with a heart defect and surgically treated. Four women aged 21-30 years were participants of this study.

[The concept of "dialogical self" and its' application for psychotherapy and especially for family therapy].

The authors show how to use the concept of a "dialogical self' to conduct therapy, supervision and qualitative research of the process of psychotherapy. They pay special attention to usefulness of the presented concept and to investigate what happens to a therapist during a family meeting, i.e.

[The concept of the "dialogical self' in psychotherapy--theoretical assumptions].

The authors present Michaił Bachtin's and Hubert Herman's concept of "dialogical self" which was used by family therapists to create a new conceptual tool of understanding the family therapy process. The paper shows Peter Rober's and his co-workers works that use this concept in order to understand phenomena occurring during therapeutic meeting.

[The need of qualitative research in psychiatry].

The authors present basic information about qualitative research along with its theoretical frame as a useful approach in the study of many issues in the area of psychiatry and psychotherapy. According to the authors, the quantitative methodology--dominant in psychiatry nowadays--seems insufficient to address all issues to be studied in this area. After a short introduction of the theoretical background of qualitative research, the authors present methods of collecting and analysing qualitative data and possible ways of using them in practice.

[Assessment of the physical development in children with short bowel syndrome based on selected anthropometric parameters].

Introduction: Short bowel syndrome (SBS) results from partial surgical resection of small bowel and requires parenteral nutrition. Aim of the study was to assess physical development and nutrition status of children with SBS.

Material And Methods: The study sample included 176 patients (56 girls and 120 boys) aged from 3 to 10 years.

Multi-couple reflecting team: preliminary report.

The aim of therapeutic help in marital crisis is to break the vicious circle of mutual hurtful accusations. The method that the authors present below involves a group of couples working within a closed cycle of meetings. In the course of successive group meetings, the various couples act as reflecting teams (RTs) for each other.

[Estimation of the correlation of insulin resistance and selected adipocytokines in children with simple obesity--preliminary study].

Background: Insulin resistance--a key element of the metabolic syndrome--is observed in children with simple obesity. Adipose tissue is producing bioactive substances called adipocytokines. Some of them may play a role in the development of insulin resistance.