Publications by authors named "Janousek L"

Objectives: Vascular access (VA) stenoses play a significant role in the morbidity of the haemodialysed population. Classifications for diagnosis, assessment and proposal of treatment strategies can be useful clinical and methodological tools. This review aims to present a comprehensive summary and propose further methodological approaches.

View Article and Find Full Text PDF

Background: Venotonics are a class of therapeutically active molecules that have vaso-protective effects. They are used to alleviate venous diseases and disorders, particularly venous insufficiency. We compared the composition of prescription versus over-the-counter (OTC) venotonics using high-performance liquid chromatography with UV detection (HPLC-DAD) and simulating their digestion using a static digestive model.

View Article and Find Full Text PDF

On the basis of author's request the publisher of Physiological Research decided to change the license of the article to CC BY license.

View Article and Find Full Text PDF
Article Synopsis
  • Recent research tested a new treatment called LIS1, derived from genetically modified pigs, to see if it could safely be used in kidney transplant patients instead of traditional rabbit-derived antithymocyte globulins (ATGs).
  • The study showed that LIS1 effectively depleted T cells without causing major side effects, such as cytokine release syndrome, and was well tolerated by patients.
  • Results indicated that LIS1 has a long half-life and allows for quick recovery of lymphocyte counts, suggesting it could be a promising alternative for transplant immunosuppression.
View Article and Find Full Text PDF

An analytical method for studying DNA degradation by electrophoresis after cell lysis and visualization of DNA fragments with fluorescent dye, comet assay, was used to evaluate the viability of the endothelial layer of human arterial grafts with the aim of identifying the procedure that will least damage the tissue before cryopreservation. Four groups of samples were studied: cryopreserved arterial grafts that were thawed in two different ways, slowly lasting 2 hours or rapidly for approx. 7 minutes.

View Article and Find Full Text PDF
Article Synopsis
  • Polyclonal rabbit antithymocyte globulins (ATGs) used in organ transplants can cause unwanted inflammation, while LIS1, a new generation of antilymphocyte globulins derived from genetically modified pigs, aims to reduce these risks.
  • A phase 1 study tested LIS1 in kidney transplant patients to assess its safety, T cell depletion effects, and pharmacokinetics.
  • Results indicated that LIS1 was well tolerated, effectively depleted T cells in certain dosage groups, and did not provoke severe side effects or the formation of antidrug antibodies.
View Article and Find Full Text PDF

Introduction: This case report describes surgical treatment of chronic mesenteric ischemia in a polymorbid patient with the history of an aorto-bifemoral bypass implant.

Case Report: The patient suffered from chronic occlusions of the mesenteric arteries. He experienced postprandial pain and significant weight loss.

View Article and Find Full Text PDF

Introduction: Retroperitoneal tumours (RTs) in adults are a rare heterogeneous group of neoplasms arising from the retroperitoneal space. RTs'clinical manifestations are nonspecific and depend on their anatomical positioning and relation with bordering structures. Our study aimed to retrospectively evaluate our patients' diagnosis, length of hospital stay, disease-free period and postoperative metastasis occurrence.

View Article and Find Full Text PDF

Replacing an infected vascular prosthetic conduit with an allograft is a possible solution of this complication given the low recurrence of infection. It is most commonly utilized for cases where the use of autologous tissue is not an option. We present the case of a 70-year-old patient who had undergone repeated vascular reconstructions in the right lower limb.

View Article and Find Full Text PDF

Uterus transplantation (UTx) is a promising treatment option for women who wish to give birth but suffer from absolute uterine factor infertility. This paper presents an interim analysis of a trial focusing on the causes, prevention, diagnosis, and management of graft thrombosis. Our team analyzed 10 cases of UTx (recipients numbered 1 to 10).

View Article and Find Full Text PDF

The pro-inflammatory status of adipose tissue (AT) has been found to be related to reverse cholesterol transport (RCT) from peritoneal macrophages. However, this finding was made in experimental models using induced peritonitis and isolated peritoneal macrophages of animals. This experimental relationship is in agreement with RCT changes in man in two extreme situations, sepsis or cardiovascular complications.

View Article and Find Full Text PDF

Introduction: Aggressive fibromatosis, also known as desmoid tumour (DT), is a locally invasive soft tissue malignancy originating from fascial planes, connective tissue, and musculoaponeurotic structures of the muscles. The symptoms greatly depend on the location and size of the tumour.

Case Report: A 68-year-old male patient without any comorbidities with a large, palpable mass in the abdomen underwent computed tomography (CT) of the abdomen during diagnostic examination in September 2017 in another centre.

View Article and Find Full Text PDF
Article Synopsis
  • Rheport is an online system designed for rheumatology that triages patient referrals to assess the likelihood of inflammatory rheumatic diseases (IRD), but its current accuracy is limited.
  • A study tested nine machine learning models using a dataset from a national rheumatology registry to improve this accuracy, with results showing that ML models significantly outperformed the existing algorithm.
  • Key factors influencing IRD classification were identified, including finger joint pain and inflammatory markers, suggesting that enhancing the system with more lab data could further improve its performance.
View Article and Find Full Text PDF
Article Synopsis
  • The study aimed to investigate the relationship between different macrophage subpopulations in arterial walls and adjacent adipose tissue in living kidney donors, focusing on early atherosclerosis.
  • Using flow cytometry and RT-PCR, researchers analyzed samples to determine how these macrophage groups were linked to cardiovascular risk factors.
  • Results showed specific macrophage subtypes were associated with cardiovascular risks, highlighting a connection between perivascular adipose tissue and the arterial wall, while visceral adipose tissue did not show the same link.
View Article and Find Full Text PDF

Background: Transjugular intrahepatic portosystemic shunt (TIPS) is a method used to decrease portal hypertension. Biliary stricture is the rarest of the complications associated with this procedure with only 12 cases previously reported in the literature. None of these cases have documented the resolution of biliary stenosis induced by a stent graft.

View Article and Find Full Text PDF

Introduction: Thrombolysis has been suggested as a feasible method to treat arterial renal transplant thrombosis under conditions of short duration of ischemia. Data on maximal duration of ischemia that are still feasible to treat are scarce.

Methods: We retrospectively analysed our experience involving three attempts to utilize thrombolysis to treat transplant renal artery thrombosis.

View Article and Find Full Text PDF

The authors present their contribution to the improvement of methods suitable for the detection of the freezing and thawing damage of cells of cryopreserved venous grafts used for lower limb revascularization procedures. They studied the post-thaw viability of cells of the wall of cryopreserved venous grafts (CVG) immediately after thawing and after 24 and 48 h culture at +37 °C in two groups of six CVG selected randomly for slow thawing in the refrigerator and rapid thawing in a water bath at +37 °C. The grafts were collected from multi-organ and tissue brain-dead donors, cryopreserved, and stored in a liquid nitrogen vapor phase for five years.

View Article and Find Full Text PDF

Objectives: The aim of this study was to investigate the association of lower urinary tract dysfunctions with urinary leakage from ureterocystoneoanastomosis (UCNA) after kidney transplantation.

Background: The UCNA leakage after kidney transplantation can be associated with various conditions while severe lower urinary tract dysfunctions could be one of them.

Methods: The analysis included all men who underwent kidney transplantation between January 2009 and December 2014.

View Article and Find Full Text PDF

Statins represent one of the most widely used classes of drugs in current medicine. In addition to a substantial decrease in atherogenic low density lipoprotein (LDL) particle concentrations, several large trials have documented their potent anti-inflammatory activity. Based on our preliminary data, we showed that statins are able to decrease the proportion of pro-inflammatory macrophages (CD14+16+CD36high) in visceral adipose tissue in humans.

View Article and Find Full Text PDF

Introduction: Uterus transplantation (UTx) is a rapidly evolving treatment of uterine-factor infertility. We report the results of the first 10 UTx procedures performed at our institution.

Methods: The program started in April 2016 as a two-arm study comparing the efficacy of UTx from live donors (LD) and deceased donors (DD).

View Article and Find Full Text PDF

Introduction: Acute early vascular complications are rare, but serious complications after kidney transplantation. They often result in graft loss. For this reason, shortening the diagnostic process is crucial.

View Article and Find Full Text PDF

Introduction: Liver transplantation is established as a lifesaving procedure for patients with acute and chronic liver failure, as well as certain selected malignancies. Due to a continuing organ shortage and ever-growing patient waiting lists, donation after cardiac death (DCD) is becoming more frequently utilized in order to close the gap between “supply and demand”.

Methods: A retrospective analysis of DCD and subsequent liver transplantations was performed.

View Article and Find Full Text PDF

Introduction: A 72-year-old male patient was admitted into our centre with large infected pseudoaneurysm (PSA) in the left groin. The patient underwent a CT angiography (CTA) that confirmed a large partly thrombosed 6.5 × 5.

View Article and Find Full Text PDF

Introduction: The rate of thawing of cryopreserved human iliac arteries allografts (CHIAA) directly affects the severeness of structural changes that occur during this process.

Method: The experiment was performed on ten CHIAA. The 10% dimethylsulphoxide in 6% hydroxyethyl starch solution was used as the cryoprotectant; all CHIAA were cooled at a controlled rate and stored in the vapor phase of liquid nitrogen (-194°C).

View Article and Find Full Text PDF

Solution of inverse problem in eddy-current non-destructive evaluation of material defects is concerned in this study. A new inverse algorithm incorporating three methods is proposed. The wavelet transform of sensed eddy-current responses complemented by the principal component analysis and followed by the neural network classification are employed for this purpose.

View Article and Find Full Text PDF