Publications by authors named "Janno B B Bernadus"
The aim of this study was to investigate the microbial diversity encompassing bacteria, fungi, and viruses within the composite microbial community associated with mosquitoes in Manado, Indonesia, using a whole-genome shotgun metagenomics approach. Female mosquitoes were collected and grouped into pools of 50 individuals, from which genomic DNA (gDNA) and RNA were extracted separately. Whole-genome shotgun metagenomics were performed on gDNA samples.
View Article and Find Full Text PDFChikungunya (CHIK) is an emerging and reemerging infectious disease of public health importance in Indonesia. Information on the asymptomatic and true burden of CHIK virus (CHIKV) infections is limited. We assayed 1,092 healthy population samples, collected in North and South Sulawesi between 2019 and 2020, for antibodies against CHIKV.
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- Researchers tested 244 patients with acute meningoencephalitis to see if there was a link between CHIKV and neurological symptoms, using various lab techniques to analyze cerebrospinal fluid and blood samples.
- The study identified four cases of possible CHIKV-related neurological disease, highlighting the need to consider CHIKV among other viral infections when diagnosing patients in endemic regions, especially during outbreaks.
Article Synopsis
- - The study examines the knowledge, attitudes, and practices (KAP) regarding zoonotic disease transmission among communities involved in wildlife trade in North Sulawesi, Indonesia, to help prevent future outbreaks.
- - Researchers employed both qualitative (interviews and focus groups) and quantitative (questionnaires) methods, sampling 477 participants for surveys and collecting biological specimens from 254 individuals in targeted areas known for wildlife consumption.
- - Findings revealed that while participants had some awareness of zoonotic diseases like rabies and bird flu, only 1% of the quantitative study group believed that contact with wild animals could lead to illness; biological tests found only one participant with evidence of a zoonotic infection.
Background: Rickettsia felis has recently emerged worldwide as a cause of human illness. Typically causing mild, undifferentiated fever, it has been implicated in several cases of non-fatal neurological disease in Mexico and Sweden. Its distribution and pathogenicity in Southeast Asia is poorly understood.
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- * A study at R.D. Kandou General Hospital in Manado involved testing samples from adults with suspected CNS infections, revealing a confirmed viral cause in three patients and probable causes in eleven.
- * The dominant virus identified was herpes simplex virus 1, which impacted 9.5% of patients; the study emphasized the need for improved diagnostic methods to detect viral CNS infections effectively.