An easy-to-perform protocol for culturing primary murine lung tumor cells as organoids.

Cancer research involves significant animal consumption and suffering. Tumor cells can be differentiated in vitro into three-dimensional organoids that resemble the primary tumor. In basic cancer research, however, tumor organoids are usually only used alongside animal experiments.

Synthesis of 4-substituted azopyridine-functionalized Ni(II)-porphyrins as molecular spin switches.

We present the synthesis and the spin switching efficiencies of Ni(II)-porphyrins substituted with azopyridines as covalently attached photoswitchable ligands. The molecules are designed in such a way that the azopyridines coordinate to the Ni ion if the azo unit is in configuration. For steric reasons no intramolecular coordination is possible if the azopyridine unit adopts the configuration.

Azo-dimethylaminopyridine-functionalized Ni(II)-porphyrin as a photoswitchable nucleophilic catalyst.

We present the synthesis and the photochemical and catalytic switching properties of an azopyridine as a photoswitchable ligand, covalently attached to a Ni(II)-porphyrin. Upon irradiation with 530 nm (green light), the azopyridine switches to the configuration and coordinates with the Ni ion. Light of 435 nm (violet) isomerizes the ligand back to the configuration, which decoordinates for steric reasons.

A metrologically traceable protocol for the quantification of trace metals in different types of microplastic.

The presence of microplastic (MP) particles in aquatic environments raised concern about possible enrichment of organic and inorganic pollutants due to their specific surface and chemical properties. In particular the role of metals within this context is still poorly understood. Therefore, the aim of this work was to develop a fully validated acid digestion protocol for metal analysis in different polymers, which is a prerequisite to study such interactions.