Publications by authors named "Janna Goltsman"
Article Synopsis
- The Integrase (Int) from coliphage HK022 enables DNA recombination in human cells between two att sites without needing accessory proteins IHF and Xis.
- Previous studies found that recombination could occur without selection when both att sites were on the same chromosome, but required selection when they were on separate vectors (in trans).
- This research demonstrates that optimizing the int-HK022 gene for human codons and adding IHF and Xis significantly enhance recombination efficiency, allowing detection of trans recombinants without the need for selection.
It has been previously demonstrated that the wild type integrase (Int) protein of coliphage HK022 can catalyze site-specific recombination in human cells between attachment (att) sites that were placed on extrachromosomal plasmids. In the present report it is shown that Int can catalyze the site-specific recombination reactions in a human cell culture on the chromosomal level. These include integrative (attP x attB) as well as excisive (attL x attR) reactions each in two configurations.
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