Efficient Somatic Embryogenesis, Regeneration and Acclimatization of Meyer: True-to-Type Conformity of Plantlets as Confirmed by ISSR Analysis.

Meyer grows in east Russia and Asia. There is a high demand for this crop due to its medicinal properties. However, its low reproductive efficiency has been a hindrance to the crop's widespread use.

Comparative Analysis of Panax ginseng Berries from Seven Cultivars Using UPLC-QTOF/MS and NMR-Based Metabolic Profiling.

The commercial use of berries is increasing as berries are known to contain large amounts of ginsenosides, and many pharmacological activities have been reported for the various ginsenosides. For the proper use of berries, it is necessary to study efficient and accurate quality control and the profiling of the overall composition of each cultivar. Ginseng berry samples from seven cultivars (Eumseung, Chung-buk Province, Republic of Korea) were analyzed using ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-QTOF/MS) for profiling of the ginsenosides, and high-resolution magic-angle-spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy for profiling of the primary metabolites.

Metagenomic analysis of bacterial endophyte community structure and functions in at different ages.

This study investigated the root-associated bacterial endophytes of at different ages by shotgun metagenomic analysis. After mapping metagenome data to the complete ginseng genome to identify unmapped sequences, we predicted the structure and functions of ginseng bacterial endophytes by metagenomic rapid annotation using subsystems technology analysis. While Proteobacteria and Actinobacteria were the predominant phyla in all samples (2-6-year-old roots), class Alphaproteobacteria was most abundant in 3-, 4-, and 5-year-old plants.

Complete Genome Sequence of the Endophytic Bacterium Bacillus cereus PgBE311, Isolated from .

Bacillus cereus PgBE311, isolated from the root tissue of a 5-year-old Panax ginseng plant, showed activities against the fungal pathogens Cylindrocarpon destructans and Botrytis cinerea. Here, we report the genome sequence of B. cereus PgBE311.

Complete Genome Sequence of the Endophytic Bacterium Chryseobacterium indologenes PgBE177, Isolated from Panax quinquefolius.

Chryseobacterium indologenes PgBE177, isolated from the root tissue of a 4-year-old Panax quinquefolius plant, showed antagonistic activity against Pseudomonas syringae pv. tomato DC3000, a bacterial pathogen. Here, we report the whole-genome sequence of C.

C/N/O/S stable isotopic and chemometric analyses for determining the geographical origin of cultivated in Korea.

Background: The geographical origin of Meyer, a valuable medicinal plant, is important to both ginseng producers and consumers in the context of economic profit and human health benefits. We, therefore, aimed to discriminate between the cultivation regions of ginseng using the stable isotope ratios of C, N, O, and S, which are abundant bioelements in living organisms.

Methods: Six Korean ginseng cultivars (3-yr-old roots) were collected from five different regions in Korea.

Diversity of bacterial endophytes in and their protective effects against pathogens.

Although endophytic bacteria are known to colonize , little is known about their diversity and roles. We addressed in the present study by comparing endophytic bacterial populations in plants of different ages (2-6 years) and in various tissue types (root, stem, leaf, and flower stalk). A total of 116 strains assigned to 42 species were identified by 16S rDNA sequencing.

A Novel Multifunctional C-23 Oxidase, CYP714E19, is Involved in Asiaticoside Biosynthesis.

Centella asiatica is widely used as a medicinal plant due to accumulation of the ursane-type triterpene saponins asiaticoside and madecassoside. The molecular structure of both compounds suggests that they are biosynthesized from α-amyrin via three hydroxylations, and the respective Cyt P450-dependent monooxygenases (P450 enzymes) oxidizing the C-28 and C-2α positions have been reported. However, a third enzyme hydroxylating C-23 remained elusive.

The mRNA and miRNA transcriptomic landscape of Panax ginseng under the high ambient temperature.

Background: Ginseng is a popular traditional herbal medicine in north-eastern Asia. It has been used for human health for over thousands of years. With the rise in global temperature, the production of Korean ginseng (Panax ginseng C.

Isoform Sequencing Provides a More Comprehensive View of the Panax ginseng Transcriptome.

Korean ginseng ( C.A. Meyer) has been widely used for medicinal purposes and contains potent plant secondary metabolites, including ginsenosides.