The objective was to evaluate development of cloned embryos constructed with serum-starved versus fed ovarian granulosa cells. Fusion of somatic cells with cytoplasts (50.7 and 50.
- The study examines how elevated temperatures affect bovine oocytes at the germinal vesicle (GV) stage, using S-roscovitine to keep the oocytes from maturing prematurely.
- Researchers cultured cumulus-oocyte complexes (COCs) at either 38.5 or 41 degrees Celsius and found that higher temperatures led to changes in yolk granule types and a reduced progression to metaphase II during maturation.
- It was concluded that elevated temperatures directly negatively impact oocyte development, contributing to lower fertility rates in heat-stressed dairy cows.