Glomerular Filtration Rate Assessment in Children.

Glomerular filtration rate (GFR) measurement is a key tool for determining the degree of chronic kidney disease. The assessment of GFR is even more challenging in children than in adults with more variables in the equation than race and sex. Monitoring the progress of the kidney disease can therefore be difficult as in the initial stages of a decline in kidney function, there are no clinical signs.

Influence of stathmin 1 (STMN1) expression on neoangiogenesis in colorectal adenocarcinoma.

Stathmin1 is a microtubular regulatory protein. The expression disorders of this protein result in significant changes in cell migration, invasion, adhesion and colony formation in many malignant tumors. The aim of our research was to investigate the effects of Stathmin1 expression on neoangiogenesis in colorectal adenocarcinoma.

Expression of endoglin, CD105, in conjunctival melanocytic nevi: Is it suspicious like in thyroidology? Oculi plus vident quam oculus?

Objective: The aim of this study was to evaluate the expression of endoglin and its correlation with histopathological and clinical findings in conjunctival nevi.

Methods: The study included archival formalin-fixed, paraffin-embedded tissue sections of 44 patients with conjunctival nevi. Immunohistochemical staining for CD105 had been performed with monoclonal mouse antihuman CD105 antibodies.

Clinical and pathological significance of proliferation index and p53 expression in gastric adenocarcinoma.

Purpose: The purpose of our work was to investigate the association between proliferative index [proIDX] and expression index p53 (p53IDX) with the clinical and pathological characteristics of gastric adenocarcinoma.

Methods: The biopsy material of 90 patients operated on for gastric cancer was routinely processed in paraffin and archived. After the histopathological report was made, two study groups were formed, the first group (n=45) comprised biopsies with intestinal carcinoma and the second (n=45) biopsies of diffuse gastric cancer.

Clinical - pathological significance of leptin receptor (LEPR) expression in squamous cell carcinoma of the skin.

Adipokine leptin functions through its transmembrane receptors (LEPR). In many malignant tumors it stimulates the growth, migration and invasion of malignant cells. The aim of our work is to examine the effect of LEPR expression on the clinical-morphological properties of squamous cell carcinoma of the skin (cSCC).

Clinical significance of neoangiogenesis and index of proliferation in the signet ring type of gastric adenocarcinoma.

Purpose: The purpose of this study was to examine whether microvascular density and the level of proliferation in gastric signet ring cell carcinoma (SRCC) are important factors in the locoregional control of the disease.

Methods: Over a period of eight years, gastric resection specimens from 37 patients were examined. The proliferative index (labelled by Ki67) and microvascular density (MVD) index (mvdIDX) (labelled by CD105) were determined for each case of SRCC.

Comparison of cytological categories atypical (C3) and suspected (C4) with histopathological diagnoses of breast lesions.

Purpose: Fine needle aspiration cytology (FNAC) is a diagnostic method characterized by high sensitivity, specificity and predictive value. In order to obtain uniformed results of FNAC breast changes, the following categories are introduced: C1 (non-representative), C2 (benign), C3 (atypical), C4 (suspected) and C5 (malignant). The purpose of this study was to establish which pathological processes are most frequently diagnosed as C3 and C4 categories, which carry a malignant tumor risk.

Vitamin B complex mitigates cardiac dysfunction in high-methionine diet-induced hyperhomocysteinemia.

This research is designed to test the hypothesis that elevated homocysteine (Hcy) levels in vivo, caused by a deficit in vitamin B complex, promote changes in cardiac function and redox status that lead to heart failure. In order to conduct the study, we used adult male Wistar albino rats (n = 30; 4 weeks old; 100 ± 15 g body weight). Hyperhomocysteinaemia (HHcy) in these animals was achieved by dietary manipulation.

Clinical significance of microvessel density and proliferation in prostate cancer core biopsy.

Purpose: To investigate the microvessel density (MVD) and proliferation in prostate cancer (PC) core biopsies.

Methods: Core biopsy samples of PC tissue from 45 patients were routinely processed and embedded in paraffin. The samples of PC formed the investigated group (n=25), while samples of benign prostatic hyperplasia (BPH) served as controls (n=20).

Production of Secondary Metabolites in Extreme Environments: Food- and Airborne Wallemia spp. Produce Toxic Metabolites at Hypersaline Conditions.

The food- and airborne fungal genus Wallemia comprises seven xerophilic and halophilic species: W. sebi, W. mellicola, W.

Cetuximab in third-line therapy of patients with metastatic colorectal cancer: A single institution experience.

Purpose: Cetuximab, an IgG1 chimeric monoclonal antibody (MAB) against epidermal growth factor receptor (EGFR) has activity against metastatic colorectal cancers (mCRC) that express EGFR. The purpose of this study was to demonstrate the efficacy and safety of cetuximab administered to patients with EGFR-positive mCRC.

Methods: 72 patients with wild-type KRAS mCRC were enrolled.

Interplay between expression of leptin receptors and mucin histochemical aberrations in colorectal adenocarcinoma.

Background: There is no information on the effects of leptin receptors expression on mucin-histochemical alterations in human colorectal adenocarcinoma.

Aim: Testing the correlation of leptin receptors expression with histochemical dysregulation of mucins in colorectal adenocarcinoma.

Patients And Methods: The study included 75 patients with colorectal adenocarcinoma who underwent surgical resection.

A Taxonomic Revision of the Wallemia sebi Species Complex.

Wallemia sebi is a xerophilic food- and air-borne fungus. The name has been used for strains that prevail in cold, temperate and tropical climates. In this study, multi-locus phylogenetic analyses, using the internal transcribed spacer (ITS) regions, DNA replication licensing factor (MCM7), pre-rRNA processing protein (TSR1), RNA polymerase II largest subunit (RPB1), RNA polymerase II second largest subunit (RPB2) and a new marker 3´-phosphoadenosine-5´-phosphatase (HAL2), confirmed the previous hypothesis that W.

The relationship between proliferation activity and parathyroid hormone levels in parathyroid tumors.

Purpose: This article examines as to whether the Ki-67 index may be useful as a marker for cell proliferation, as well as to whether Ki-67 immunohistochemical expression and parathyroid hormone (PTH) levels are useful in distinguishing between parathyroid carcinoma (PC) and adenoma.

Methods: A retrospective analysis of 50 patients (10 with PC and 40 with adenoma) who had been previously diagnosed with primary hyperparathyroidism (PHPT) was conducted. Normal parathyroid glands served as the control group.

Application of the phylogenetic species concept to Wallemia sebi from house dust and indoor air revealed by multi-locus genealogical concordance.

A worldwide survey of Wallemia occurring in house dust and indoor air was conducted. The isolated strains were identified as W. sebi and W.

Involvement of leptin receptors expression in proliferation and neoangiogenesis in colorectal carcinoma.

Purpose: This study tested whether there exists a correlation between leptin receptors (LEPR) expression with proliferation and neoangiogenesis in colorectal carcinoma.

Methods: Enrolled were 75 patients with colorectal carcinoma, who underwent surgical tumor resection. After routine histopathological preparation, sections 3-4 μm thick were prepared.

Expression of vascular endothelial growth factor and microvascular density assessment in different histotypes of basal cell carcinoma.

Purpose: In this study, we investigated the expression of vascular endothelial growth factor (VEGF) and tumor microvascular density (MVD) in different histotypes of basal cell skin carcinoma (BCC).

Methods: We used a total 101 histological archival specimens, including superficial, nodular, cystic, keratinocytic, adenoid infiltrative types and cases of metatypical BCC. Routine hematoxylin/eosin (H&E) and immunohistochemical ABC method with NOT AE1/AE3, anti VEGF anti CD34 antibodies were used.

Difference between Luminal A and Luminal B Subtypes According to Ki-67, Tumor Size, and Progesterone Receptor Negativity Providing Prognostic Information.

Background: The St. Gallen International Expert Consensus of 2011 proposes a new classification system for breast cancer based on its division into five subgroups. The criteria to identify these subtypes were recently refined at the 2013 Conference.

Cadmium effects on the thyroid gland.

Cadmium has been listed as one of the 126 priority pollutants and a category I carcinogen. Carcinogenic effects of cadmium on the lungs, testicles, and prostate are widely recognized, but there has been insufficient research on the effect of cadmium on the thyroid gland. Cadmium has the affinity to accumulate not only in the liver, kidneys, and pancreas but also in the thyroid gland.

Relationship of E-cadherin with cervical lymph node metastasis in laryngeal cancer.

E-cadherin, a 120 kDa transmembrane protein, plays an important role in malignant progression and tumour differentiation. The loss or reduction in E-cadherin expression has been found in several tumours including laryngeal squamous cell carcinoma. The present study aimed to investigate the prognostic implications of changes in expression of the E-cadherin in laryngeal carcinoma.

Quantitative evaluation of stromal myofibroblasts and their significance for the metastatic capacity of colorectal carcinoma.

Purpose: To quantify the myofibroblasts in the tumor stroma of colorectal carcinomas using immunostaining with anti smooth muscle actin (SMA) as a marker for myofibroblasts.

Methods: The study was carried out on 46 surgically resected primary colorectal adenocarcinomas from the archive of the Centre for Pathology and Forensic Medicine of the Military Medical Academy in Belgrade, from 2008-2010. All samples were analysed by the scientific software "Image J".

Focal neuroendocrine differentiation in prostatic gland carcinoma with basaloid pattern.

Introduction: Prostatic gland basal cell proliferations exhibit morphological continuum ranging from basal cell hyperplasia to basal cell carcinoma. In the following report, we described clinical features, morphological spectrum, neuroendocrine differentiation and histogenesis of prostatic gland basal cell carcinoma in our patient.

Case Report: Hematoxylin-eosin (HE), Alcian blu-periodic acid schiff (AB-PAS) at pH 2.

Calcitonin gene-related peptide (CGRP) - microadenomas of the thyroid gland induced by cadmium toxicity. Experimental study.

Purpose: The objective of our study was to make morphological and functional analysis of thyroid C cells in rats chronically exposed to cadmium (Cd).

Methods: The study was carried out on female albino Wistar rats (n=22, age=35-37 days, body mass 120-140 g), divided in control (n=11) and experimental group (n=11). The rats of the experimental group were treated with 15 mg/kg Cd dissolved (as CdCl(2)) in drinking water.