Resolving spatiotemporal electrical signaling within the islet via CMOS microelectrode arrays.

Glucose-stimulated beta-cells exhibit synchronized calcium dynamics across the islet that recruit beta-cells to enhance insulin secretion. Compared to calcium dynamics, the formation and cell-to-cell propagation of electrical signals within the islet are poorly characterized. To determine factors that influence the propagation of electrical activity across the islet underlying calcium oscillations and beta-cell synchronization, we used high-resolution CMOS multielectrode arrays (MEA) to measure voltage changes associated with the membrane potential of individual cells within intact C57BL6 mouse islets.

A novel fluorescent labeling compound for GluN2A containing -methyl-d-aspartate receptors identified by autodisplay-based screening.

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