Publications by authors named "Jan uitdeWilligen"

The method of graphical genotyping is applied to a panel of tetraploid potato cultivars to visualize haplotype sharing. The method allowed to map genes involved in virus and nematode resistance. The physical coordinates of the amount of linkage drag surrounding these genes are easily interpretable.

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A 20K SNP array was developed and a comprehensive set of tetraploid cultivar was genotyped. This allowed us to identify footprints of the breeding history in contemporary breeding material such as identification of introgression segments, selection and founder signatures. A non-redundant subset of 15,138 previously identified SNPs and 4454 SNPs originating from the SolCAP project were combined into a 20k Infinium SNP array for genotyping a total of 569 potato genotypes.

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Article Synopsis
  • - The study focuses on assessing genomic DNA variations and calling genotypes in autotetraploid potato cultivars using a method called genotyping-by-sequencing (GBS), which was enhanced through in-solution hybridisation for more effective analysis of specific genes.
  • - A total of 12.4 Gigabases of high-quality sequence data were generated from 83 potato cultivars, leading to the identification of 129,156 sequence variants with significant nucleotide diversity, and a considerable portion of these variants were rare.
  • - The findings included confirmation of the results through KASP genotyping assays, indicating that read depths of around 60-80× are adequate for accurately estimating allele copy numbers in autotetraploids. *
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We have investigated the genetics and molecular biology of orange flesh colour in potato (Solanum tuberosum L.). To this end the natural diversity in three genes of the carotenoid pathway was assessed by SNP analyses.

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Background: Utilization of the natural genetic variation in traditional breeding programs remains a major challenge in crop plants. The identification of candidate genes underlying, or associated with, phenotypic trait QTLs is desired for effective marker assisted breeding. With the advent of high throughput -omics technologies, screening of entire populations for association of gene expression with targeted traits is becoming feasible but remains costly.

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Carotenoids are important lipophilic antioxidants in fruits. Apocarotenoids such as alpha-ionone and beta-ionone, which are breakdown products of carotenoids, are important for the flavor characteristics of raspberry fruit, and have also been suggested to have beneficial effects on human health. Raspberry is one of the few fruits where fruit ripening is accompanied by the massive production of apocarotenoids.

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