A three-phase microfluidic chip for rapid sample clean-up of alkaloids from plant extracts.

A three-phase microchip was developed for the rapid and efficient small-scale purification of alkaloids from plant extracts. As part of the development of such a three-phase microchip, first a two-phase microchip with two channels (3.2 cm and 9.

Comparison of two-phase lipase-catalyzed esterification on micro and bench scale.

Lipase type B from Candida antarctica was used to catalyze the esterification of propionic acid and 1-butanol in a water/n-decane two-phase system on micro and on bench scale. The reaction was described by a Ping Pong Bi Bi mechanism with alcohol inhibition. The kinetic parameters on micro and bench scale were compared; no significant differences were found.