Sites of biosynthesis, distribution and phloem transport of 3-isobutyl-2-Methoxypyrazine in Capsicum annuum (bell pepper) plants.

The distribution and translocation as well as the biosynthetic sites of 3-isobutyl-2-methoxypyrazine (IBMP) in bell pepper plants (Capsicum annuum) were examined. For IBMP-quantification, a stable-isotope dilution assay (SIDA) was developed, using headspace solid-phase microextraction (HS-SPME) combined with gas chromatography and time-of-flight mass spectrometry (ToF-MS). While IBMP was the most abundant MP in all aerial plant parts, IPMP was dominating in root tissues.