Publications by authors named "Jan O Wirth"
Dynein is the primary molecular motor responsible for retrograde intracellular transport of a variety of cargoes, performing successive nanometer-sized steps within milliseconds. Due to the limited spatiotemporal precision of established methods for molecular tracking, current knowledge of dynein stepping is essentially limited to slowed-down measurements in vitro. Here, we use MINFLUX fluorophore localization to directly track CRISPR/Cas9-tagged endogenous dynein with nanometer/millisecond precision in living primary neurons.
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- Neurons use motor proteins to transport materials across long neurites, which requires precise movement and tracking at nanometer resolution.
- The study focuses on a mutated kinesin-1 protein in rat hippocampal neurons, exploring its behavior as it moves along microtubules.
- Using MINFLUX technology, the researchers identified specific movements and binding states of kinesin-1, revealing its ability to switch microtubules during transport.
We introduce an interferometric MINFLUX microscope that records protein movements with up to 1.7 nanometer per millisecond spatiotemporal precision. Such precision has previously required attaching disproportionately large beads to the protein, but MINFLUX requires the detection of only about 20 photons from an approximately 1-nanometer-sized fluorophore.
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