Nile red staining for rapid screening of plastic-suspect particles in edible seafood tissues.

Concerns regarding microplastic (MP) contamination in aquatic ecosystems and its impact on seafood require a better understanding of human dietary MP exposure including extensive monitoring. While conventional techniques for MP analysis like infrared or Raman microspectroscopy provide detailed particle information, they are limited by low sample throughput, particularly when dealing with high particle numbers in seafood due to matrix-related residues. Consequently, more rapid techniques need to be developed to meet the requirements of large-scale monitoring.

Physicochemical and Sensory Characterization of Whey Protein-Enriched Semihard Cheese.

In view of potential future changes of German food legislation with regard to cheese product quality parameters, this study aimed to evaluate the quality of whey protein-enriched semihard cheese (WPEC). Model WPEC was produced in a pilot plant and on an industrial scale by adding defined amounts of high-heat (HH) milk to the cheese milk and comprehensively analyzed during cheese processing. The dry matter, total protein, pure protein, fat, and sodium chloride content of six-week ripened cheese samples were not significantly different ( < 0.

Fast and User-Friendly Detection of Flatfish Species ( and ) via Loop-Mediated Isothermal Amplification (LAMP).

The detection of a Cytochrome gene () for species differentiation in fish is intensively used. A fast alternative to expensive and time-consuming DNA barcoding is loop-mediated isothermal amplification (LAMP) in combination with efficient readout systems. For this reason, we developed LAMP assays for rapid species detection of and , two economically important flatfish species in Europe that are prone to mislabeling.

Identification of Marker Peptides for the Whey Protein Quantification in Edam-Type Cheese.

Several technologies are available for incorporating whey proteins into a cheese matrix. However, there is no valid analytical method available to determine the whey protein content in matured cheese, to date. Consequently, the aim of the present study was to develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of individual whey proteins based on specific marker peptides ('bottom-up' proteomic approach).

Uridine as a non-toxic actinometer for UV-C treatment: influence of temperature and concentration.

UV-C treatment is an effective method to inactivate microorganisms and therefore gets increasingly more attention in food industry, especially for liquid products. To test and monitor different UV-C reactor designs, a photochemical actinometer is required that gives reliable UV-C dose values and is non-toxic allowing frequent control of the production chain. Here, a variable concentrated aqueous uridine solution is tested as a photochemical actinometer.

Objectively measured adherence to physical activity among patients with coronary artery disease: Comparison of the 2010 and 2020 World Health Organization guidelines and daily steps.

Background: Tailored recommendations for patients after percutaneous coronary interventions (PCI) need physical activity (PA) to be objectively measured and assessed for adherence to guidelines. The recent WHO guidelines removed the daily recommended bout duration, while the potential impact of this change on patients after PCI remains unclear.

Aim: We evaluated prevalence estimates of adherence to PA recommendations among patients after PCI across the 2010 [≥30 min moderate- to vigorous-intensity PA (MVPA) at ≥ 10-min bout duration] and 2020 WHO guidelines (≥30 min of MVPA of any bout duration), as well as 7,500 and 10,000 steps.

Influence of processing and storage on the iodine content of meat and fish products using iodized salt.

Table salt fortified with KIO is commonly used to prevent iodine deficiency disorders. However, there is a lack of reliable data about the stability of KIO during food processing. In this study several meat and fish products were prepared with iodized salt and the iodine stability was determined through the whole production process.

High-Performance Thin-Layer Chromatography-Immunostaining as a Technique for the Characterization of Whey Protein Enrichment in Edam Cheese.

Whey protein-enriched cheese can be produced by means of a high-temperature treatment of a part of the cheese milk. In this way, the nutritional quality of the resulting cheeses can be increased while resources are conserved. High-performance thin-layer chromatography-immunostaining (HPTLC-IS) using specific β-lactoglobulin (β-LG) antibodies was applied to study the implementation and stability of β-LG in two different sample sets of whey protein-enriched Edam model cheeses, including industrial-scale ones.

Two-dimensional high-performance thin-layer chromatography for the characterization of milk peptide properties and a prediction of the retention behavior - a proof-of-principle study.

High-performance thin-layer chromatography (HPTLC) is a suitable method for the analysis of peptides and proteins due to a wide selection of stationary and mobile phases and various detection options. Especially, two-dimensional HPTLC (2D-HPTLC) enables a higher resolution compared to one-dimensional HPTLC in the separation of complex peptide mixtures. Similar to 2D electrophoresis, characteristic peptide patterns can be obtained, allowing a differentiation of ingredients based on varying protein origins.

A DNA microarray for the authentication of giant tiger prawn (Penaeus monodon) and whiteleg shrimp (Penaeus (Litopenaeus) vannamei): a proof-of-principle.

This proof-of-principle study describes the development of a rapid and easy-to-use DNA microarray assay for the authentication of giant tiger prawns and whiteleg shrimp. Following DNA extraction and conventional end-point PCR of a 16S rDNA segment, the PCR products are hybridised to species-specific oligonucleotide probes on DNA microarrays located at the bottom of centrifuge tubes (ArrayTubes) and the resulting signal patterns are compared to those of reference specimens. A total of 21 species-specific probes were designed and signal patterns were recorded for 47 crustacean specimens belonging to 16 species of seven families.

Effect of reduction of sodium content on the microbial ecology of Edam cheese samples.

Sodium intake is a major risk factor for non-communicable diseases. Consequently, reformulation of cheeses such as Edam to contain less sodium may contribute to lowering disease risk. However, sodium is essential for cheese manufacture, influencing starter culture bacteria activity and abundance during fermentation.

Developing an Automatic Color Determination Procedure for the Quality Assessment of Mangos () Using a CCD Camera and Color Standards.

Color is one of the key sensory characteristics in the evaluation of the quality of mangos () especially with regard to determining the optimal level of ripeness. However, an objective color determination of entire fruits can be a challenging task. Conventional evaluation methods such as colorimetric or spectrophotometric procedures are primarily limited to a homogenous distribution of the color.

Microbial composition of sweetness-enhanced yoghurt during fermentation and storage.

The reformulation of dairy products to contain less added sugar can contribute to reducing sugar consumption, thereby reducing the risk of non-communicable diseases. The objective of this study was to investigate the microbial ecology of reformulated yoghurt, which was produced using bi-enzymatic modification of lactose to increase its sweetness by a factor of 2-3. Ultimately, this reformulation strategy could reduce the amount of added sugar needed for equal sweetness of the end product.

[Fix the Supply Side! Demand Stimulus Will Not Cure What Ails].

The explosion of Covid-19 cases is looming in Germany. The German Society for Epidemiology has warned that the number of cases could soon overshoot the capacity of the healthcare system. This may be true even if Germany follows the 'flatten-the-curve'- approach to reduce infection rates.

Design of a user-friendly and rapid DNA microarray assay for the authentication of ten important food fish species.

Seafood is particularly susceptible to the substitution of species. In order to guarantee authentic seafood products, seafood processors and traders must perform self-checks on the authenticity of imported and purchased goods. However, the conventional Sanger sequencing of PCR products for the authentication of seafood species is time-consuming and requires advanced infrastructure.

Correction to: Chemometric tools for the authentication of cod liveroil based on nuclear magnetic resonance and infrared spectroscopy data.

The article Chemometric tools for the authentication of cod liver oil based on nuclear magnetic resonance and infrared spectroscopy data, written by Editha Giese, Sascha Rohn and Jan Fritsche.

Chemometric tools for the authentication of cod liver oil based on nuclear magnetic resonance and infrared spectroscopy data.

Cod liver oil is a popular dietary supplement marketed as a rich source of omega-3 fatty acids as well as vitamins A and D. Due to its high market price, cod liver oil is vulnerable to adulteration with lower priced vegetable oils. In this study, H and C nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, and gas chromatography (coupled to a flame ionization detector) were used in combination with multivariate statistics to determine cod liver oil adulteration with common vegetable oils (sunflower and canola oils).

Recent Developments and Digital Perspectives in Food Safety and Authenticity.

Food safety is of fundamental importance for the food processing industry, food retailers and distributors, and competent authorities because of its potentially direct impact on the health of consumers. Next to the prevention of microbiological, chemical, and physical hazards, increasing efforts are currently made to combat risks associated with food fraud or food authenticity. Food safety management systems nowadays comprise food safety, food defense, and food fraud prevention measures, trying to cope with the increasing complexity and globalization of the food supply chains.

Determining quality parameters of fish oils by means of H nuclear magnetic resonance, mid-infrared, and near-infrared spectroscopy in combination with multivariate statistics.

Fish oil is becoming increasingly popular as a dietary supplement as well as for its use in animal feed, which is mainly due to its high contents of the health promoting omega-3 fatty acids. However, these polyunsaturated fatty acids are highly susceptible to oxidation, which results in a decrease of the fish oil quality. This study investigated the potential of H NMR, FT-MIR, and FT-NIR spectroscopy in the quality assessment of fish oils.

Mitigation strategies for ester bound 2-/3-MCPD and esterified glycidol in pre-fried breaded and frozen fish products.

Pre-frying of chloride-containing raw materials (e.g., breaded frozen fish products) can lead to the formation of fatty acid esters of 2-monochloropropane-1,3-diol, 3-monochloropropane-1,2-diol (MCPD-E), and glycidol (G-E).

Assessment of the oxidative stability of conventional and high-oleic sunflower oil by means of solid-phase microextraction-gas chromatography.

Headspace-solid-phase microextraction-gas chromatography (HS-SPME-GC) was used to identify in total 74 volatile lipid oxidation compounds altogether in thermally stressed conventional and high-oleic sunflower (HOSF) oil samples (in accelerated storage conditions for 14 days at 80°C). Out of the volatile compounds identified, six volatile compounds were selected as marker compounds for the assessment of lipid oxidation of sunflower (SF) and HOSF oils due to their low odour threshold values and fatty-rancid odour impression. Additionally, other oxidation parameters such as fatty acid composition, peroxide value (PV), anisidine value and tocopherol and tocotrienol composition were determined.

Attitudes and perceptions of AIDS clinical trials group site coordinators on HIV clinical trial recruitment and retention: a descriptive study.

HIV-seropositive blacks, Hispanics, women of all ethnicities, and injection drug users (IDUs) have low rates of clinical trial participation. The opinions of research nurses and study coordinators as potential facilitators and barriers to access to clinical trials may contribute to this disparity. Study coordinators and research nurses from the adult AIDS Clinical Trials Group (ACTG) clinical trials units responded to an anonymous computer-based survey comprising multiple choice questions and clinical scenarios.

Gas chromatographic analysis of infant formulas for total fatty acids, including trans fatty acids.

Twelve powdered and 13 liquid infant formulas were analyzed by using an extension of AOAC Official Method 996.01 for fat analysis in cereal products. Samples were hydrolyzed with 8 N HCl and extracted with ethyl and petroleum ethers.