The BR-body proteome contains a complex network of protein-protein and protein-RNA interactions.

Bacterial ribonucleoprotein bodies (BR-bodies) are non-membrane-bound structures that facilitate mRNA decay by concentrating mRNA substrates with RNase E and the associated RNA degradosome machinery. However, the full complement of proteins enriched in BR-bodies has not been defined. Here, we define the protein components of BR-bodies through enrichment of the bodies followed by mass spectrometry-based proteomic analysis.

Highly Photostable and Two-Photon Active Quantum Dot-Polymer Multicolor Hybrid Coacervate Droplets.

Fabrication and precise control of the physicochemical properties of multifunctional organic-inorganic hybrid nanocomposites find great importance in various research fields. Herein, we report the fabrication of a new class of luminescent hybrid coacervate droplets from CdTe quantum dots (QDs) and a poly(diallyldimethylammonium chloride) (PDADMAC) aqueous mixture. The colloidal stability of these droplets has been explored over wide ranges of composition, pH, and ionic strength.

Surfactant-Induced Self-Assembly of CdTe Quantum Dots into Multicolor Luminescent Hybrid Vesicles.

Here, we report the interaction of mercaptosuccinic acid (MSA)-capped CdTe quantum dots (QDs) with hexadecyltrimethylammonium bromide (CTAB) surfactant and subsequent formation of self-assembled multicolor luminescent vesicles in aqueous medium. A continuous phase sequence from clear (C1) to turbid (T1), precipitate (P), turbid (T2), and clear (C2) has been observed for QD solution upon increasing the concentration of positively charged CTAB, indicating dynamic equilibrium between various self-assembled supramolecular structures. In contrast, no such changes have been observed in the presence of negatively charged sodium dodecyl sulfate and neutral Triton X-100 surfactants, indicating specific electrostatic interactions behind the observed phase separation behavior.

Tuning of resonance energy transfer from 4',6-diamidino-2-phenylindole to an ultrasmall silver nanocluster across the lipid bilayer.

Herein we have developed a simple liposome-based donor-acceptor system across the lipid bilayer using 4',6-diamidino-2-phenylindole (DAPI) as the donor and an ultrasmall ligand-capped silver nanocluster (Ag NC) as the acceptor. The process of Förster resonance energy transfer (FRET) between DAPI and the Ag NC across the liposome bilayer has been demonstrated using steady-state and time-resolved fluorescence spectroscopy. The synthesized Ag NCs with a majority of Ag and Ag cores have been characterized using FTIR, mass spectrometry, HRTEM, UV-Vis and PL spectroscopy.

Interplay among Electrostatic, Dispersion, and Steric Interactions: Spectroscopy and Quantum Chemical Calculations of π-Hydrogen Bonded Complexes.

Ï€-Hydrogen bonding interactions are ubiquitous in both materials and biology. Despite their relatively weak nature, great progress has been made in their investigation by experimental and theoretical methods, but this becomes significantly more complicated when secondary intermolecular interactions are present. In this study, the effect of successive methyl substitution on the supramolecular structure and interaction energy of indoleâ‹…â‹…â‹…methylated benzene (indâ‹…â‹…â‹…n-mb, n=1-6) complexes is probed through a combination of supersonic jet experiments and benchmark-quality quantum chemical calculations.

Experimental observation of structures with subtle balance between strong hydrogen bond and weak n → π(*) interaction: Gas phase laser spectroscopy of 7-azaindole⋯fluorosubstituted pyridines.

In this study, interplay between a strong hydrogen bond and a very weak n → π(*) interaction has been probed through experiment for the first time. We have used resonant 2-photon ionization, Infrared-ultraviolet double resonance spectroscopy, and quantum chemistry calculation to determine the structures of 7-azaindole⋯2,6-difluoropyridine and 7-azaindole⋯2,3,5,6-tetrafluororpyridine complexes, which are stabilized by both hydrogen bonding and n → π(*) interaction. The structures of the complexes studied in the present work have been compared with the double hydrogen bonded (N-H⋯N and C-H⋯N) planar structure of 7-azaindole⋯2-fluoropyridine.