Quantifying in vitro B. anthracis growth and PA production and decay: a mathematical modelling approach.

Protective antigen (PA) is a protein produced by Bacillus anthracis. It forms part of the anthrax toxin and is a key immunogen in US and UK anthrax vaccines. In this study, we have conducted experiments to quantify PA in the supernatants of cultures of B.

Transcription of the singed-weak mutation of Drosophila melanogaster: elimination of P-element sequences by RNA splicing and repression of singed transcription in a P genetic background.

The dysgenesis-induced, hypermutable singed-weak allele has two incomplete P-elements inserted in a head-to-head configuration in the 5' non-coding exon of the singed bristle locus of Drosophila melanogaster. In the presence of P transposase, each element excises to produce single element derivatives, singed-extreme and singed-(+), that have either an extreme bristle or wild-type phenotype, respectively. In an M background, pseudo-wild-type transcripts are made that initiate at the singed promoter, read through the insertions, and are spliced to remove the P-element sequences and part of the 5' exon.