Publications by authors named "James W Blankenship"
Article Synopsis
- A new strategy for identifying covalent modification sites, particularly acetylation and methylation of histone H3 from chicken erythrocytes, has been developed using low enzyme ratios and short digestion times alongside advanced mass spectrometry techniques.
- High-accuracy MALDI-TOF mass measurements have successfully differentiated between methylated and acetylated peptides, pinpointing specific lysines on histone H3 that are modified.
- Notably, the method has revealed a new methylation site at lysine 79, and offers a faster and more efficient alternative to traditional protein analysis techniques like sequencing and Western blotting, promising advancements in chromosome research.
