The effect of anti-lymphocytic IgG on unsensitized and sensitized spleen cells in vivo and in vitro.

Rat spleen cells sensitized to bovine serum albumin and treated with anti-lymphocytic IgG are unable to transfer secondary immunological (humoral) responsiveness to irradiated isogeneic rats. Similar treatment of unsensitized spleen cells has a significant, though less marked effect, upon their ability to confer primary humoral responsiveness to irradiated recipients.

Further studies on the effect of anti-lymphocytic antibody on humoral antibody formation.

The effect of 3-, 6- and 12-day courses of anti-lymphocytic IgG treatment (1 ml of 2 g/100 ml) on the primary humoral response of random bred Wistar rats has been investigated. The primary response to alum precipitated bovine serum albumin was suppressed by all these courses. In contrast however the primary response to sheep erythrocytes was suppressed by the 6-day treatment alone or by a 3-day course augmented with azathioprine treatment.