Publications by authors named "James Sebestyen"
Article Synopsis
- The study aimed to evaluate the reliability and reproducibility of short tandem repeat (STR) profiling in low template DNA (LT-DNA) using strict testing and interpretation protocols.
- DNA samples were analyzed using a sensitive quantitative PCR method, with findings confirming that even low quantities of DNA (down to 25 pg) can produce consistent and accurate allele detection, despite challenges like stochastic effects.
- The researchers developed strict guidelines for interpreting allele data, resulting in successful and accurate DNA profiling for both non-probative and forensic case samples, demonstrating that the proposed methods are effective for forensic applications.
Aim: To develop an automated, high throughput extraction protocol in order to produce database eligible profiles from fingerprints and other low copy number (LCN) DNA sources.
Methods: Extraction of either purified control DNA or buccal cells, for example, with commercial kits was compared to extraction with a simple digestion buffer and a subsequent concentration and purification. Results were evaluated based on the amount of DNA recovered and the completeness of the DNA profiles produced.