Preparation, characterization and biological evaluation of curcumin loaded alginate aldehyde-gelatin nanogels.

Curcumin, a natural polyphenol exhibits chemopreventive and chemotherapeutic activities towards cancer. In order to improve the bioavailability and therapeutic efficacy, curcumin is encapsulated in alginate aldehyde-gelatin (Alg Ald-Gel) nanogels. Alginate aldehyde-gelatin nanogels are prepared by inverse miniemulsion technique.

Acute Carpal Tunnel Syndrome: A Review of Current Literature.

Acute carpal tunnel syndrome is a progressive median nerve compression leading to loss of two-point discrimination. Most cases encountered are in the emergency department following wrist trauma and distal radius fractures. Although rare, atraumatic etiologies have been reported and diligent evaluation of these patients should be performed.

Polyelectrolyte complex nanoparticles from cationised gelatin and sodium alginate for curcumin delivery.

Self assembled hybrid polyelectrolyte complex (PEC) nanoparticles are prepared from cationically modified gelatin and sodium alginate (Alg) by electrostatic complexation between the polymers. Cationised gelatin (CG) is prepared by the reaction of gelatin with ethylenediamine. Structural changes in gelatin, after modification with ethylenediamine are investigated by XRD and (1)H NMR spectroscopy.

Galactosylated alginate-curcumin micelles for enhanced delivery of curcumin to hepatocytes.

Galactosylated alginate-curcumin conjugate (LANH2-Alg Ald-Cur) is synthesized for targeted delivery of curcumin to hepatocytes exploiting asialoglycoprotein receptor (ASGPR) on hepatocytes. The synthetic procedure includes oxidation of alginate (Alg), modification of lactobionic acid (LA), grafting of targeting group (modified lactobinic acid, LANH2) and conjugation of curcumin to alginate. Alginate-curcumin conjugate (Alg-Cur) without targeting group is also prepared for the comparison of properties.

Microgravity as a means to incorporate HepG2 aggregates in polysaccharide-protein hybrid scaffold.

Tissue culture under microgravity provides a venue which promotes cell-cell association while avoiding the detrimental effects of high shear stress. Hepatocytes cultured on carriers or entrapped within matrices under simulated microgravity conditions showed improved cell function and proliferation. In the present study, a new approach was adopted where a non-cell adherent scaffold was incorporated with hepatospheroids (HepG2) under microgravity.

Gum arabic-curcumin conjugate micelles with enhanced loading for curcumin delivery to hepatocarcinoma cells.

Curcumin is conjugated to gum arabic, a highly water soluble polysaccharide to enhance the solubility and stability of curcumin. Conjugation of curcumin to gum arabic is confirmed by (1)H NMR, fluorescence and UV spectroscopy studies. The conjugate self assembles to spherical nano-micelles (270 ± 5 nm) spontaneously, when dispersed in aqueous medium.

Galactosylated pullulan-curcumin conjugate micelles for site specific anticancer activity to hepatocarcinoma cells.

Galactosylated pullulan-curcumin conjugate (LANH2-Pu Ald-Cur SA) is developed for target specific delivery of curcumin to hepatocarcinoma cells by five step synthetic strategy, which includes oxidation of pullulan (Pu Ald), introduction of amino group to the targeting ligand (LANH2), grafting of the LANH2 to Pu Ald, modification of curcumin (Cur SA) and conjugation of Cur SA to pullulan. Nongalactosylated pullulan-curcumin conjugate (Pu-Cur SA) is also prepared to compare the enhancement in cytotoxicity offered by the targeting group. Both LANH2-Pu Ald-Cur SA and Pu-Cur SA conjugates could self assemble to micelle in water with hydrodynamic diameters of 355±9nm and 363±10nm, respectively.

Preparation and characterisation of gelatin-gum arabic aldehyde nanogels via inverse miniemulsion technique.

Gelatin-gum arabic aldehyde nanogels designed by a nanoreactor concept using inverse miniemulsion technique were reported. Stable separate miniemulsions were prepared from gelatin (Gel) and gum arabic aldehyde (GAA). These emulsions were intermixed under sonication to obtain cross-linked nanogels.

Nanogels based on alginic aldehyde and gelatin by inverse miniemulsion technique: synthesis and characterization.

Nanogels were developed from alginic aldehyde and gelatin by an inverse miniemulsion technique. Stable inverse miniemulsions were prepared by sonication of noncontinuous aqueous phase (mixture of alginic aldehyde and gelatin) in a continuous organic phase (Span 20 dissolved in cyclohexane). Cross-linking occurred between alginic aldehyde (AA) and gelatin (gel) in the presence of borax by Schiff's base reaction during the formation of inverse miniemulsion.

Modified gum arabic cross-linked gelatin scaffold for biomedical applications.

The present work deals with development of modified gum arabic cross-linked gelatin scaffold for cell culture. A new biocompatible scaffold was developed by cross-linking gelatin (Gel) with gum arabic, a polysaccharide. Gum arabic was subjected to periodate oxidation to obtain gum arabic aldehyde (GAA).

Is radiographic evaluation necessary in children with a clinical diagnosis of calcaneal apophysitis (sever disease)?

Background: Calcaneal apophysitis (Sever disease) is most often diagnosed clinically, and radiographic evaluation is believed to be unnecessary by many physicians. To evaluate the need for radiographic evaluation in children with a clinical diagnosis of calcaneal apophysitis, we determined the frequency of abnormal radiographic findings in a group of patients with this clinical diagnosis.

Methods: Clinical records and radiographs of all children between the age of 4 and 17 years who presented with a chief complaint of heel pain were retrospectively reviewed.