Analytical and clinical validation of the 25 OH vitamin D assay for the LIAISON automated analyzer.

Objective: Methods to assess serum 25 OH vitamin D have improved in accuracy, precision, and ease of use. We describe the analytical and clinical validation of an automated, antibody- and microparticle-based, chemiluminescent immunoassay method for the determination of 25 OH vitamin D.

Design And Methods: The LIAISON 25 OH Vitamin D assay is a rapid automated method with first results available in 40 min, and a subsequent throughput of 180 samples per hour.

Analytical and clinical validation of a radioimmunoassay for the measurement of 1,25 dihydroxy vitamin D.

Objectives: The analytical and clinical validation of the DiaSorin 1,25 dihydroxyvitamin D RIA is described.

Design And Methods: The analytical parameters assessed included analytical sensitivity, dilution linearity, intra- and inter-assay precision, recovery, specificity, and interference studies. Where appropriate, assessments were performed according to NCCLS guidelines.