Publications by authors named "James Bryant Collins"
Molecular evidence of widespread benzimidazole drug resistance in Ancylostoma caninum from domestic dogs throughout the USA and discovery of a novel β-tubulin benzimidazole resistance mutation.
Abhinaya Venkatesan Pablo D Jimenez Castro Arianna Morosetti Hannah Horvath Rebecca Chen James Bryant Collins
PLoS Pathog
March 2023
Ancylostoma caninum is an important zoonotic gastrointestinal nematode of dogs worldwide and a close relative of human hookworms. We recently reported that racing greyhound dogs in the USA are infected with A. caninum that are commonly resistant to multiple anthelmintics.
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- Whole-genome sequencing is increasingly used to study helminth genomes, focusing on genome assembly, population genetics, and diagnostics, but adult parasites are difficult to access for DNA sampling.
- The study explores five low-input DNA extraction methods along with a sequencing protocol to enable whole-genome sequencing of immature helminth stages, which typically yield low DNA quantities and face contamination issues.
- Results showed successful sample preparations for most tested species, indicating that whole-genome sequencing of individual helminth parasites is feasible and can enhance diagnostic and surveillance efforts.