Publications by authors named "James Boot"

Background: Platelet function is driven by the expression of specialized surface markers. The concept of distinct circulating subpopulations of platelets has emerged in recent years, but their exact nature remains debatable.

Objectives: To design a spectral flow cytometry-based phenotyping workflow to provide a more comprehensive characterization, at a global and individual level, of surface markers in resting and activated healthy platelets, and to apply this workflow to investigate how responses differ according to platelet age.

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We describe a subset of glioblastoma, the most prevalent malignant adult brain tumour, harbouring a bias towards hypomethylation at defined differentially methylated regions. This epigenetic signature correlates with an enrichment for an astrocytic gene signature, which together with the identification of enriched predicted binding sites of transcription factors known to cause demethylation and to be involved in astrocytic/glial lineage specification, point to a shared ontogeny between these glioblastomas and astroglial progenitors. At functional level, increased invasiveness, at least in part mediated by SRPX2, and macrophage infiltration characterise this subset of glioblastoma.

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Article Synopsis
  • Scientists are studying how some special processes in brain cell development, called epigenetics, are linked to changes in a type of brain cancer called glioblastoma (GBM).
  • They developed a way to compare cells from glioblastoma with special brain stem cells from patients to find important differences.
  • This research helps identify new ways to treat GBM by finding specific genes that can be targeted with drugs based on each patient's unique cancer.
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