Targeted Delivery and Redox Activity of Folic Acid-Functionalized Nanoceria in Tumor Cells.

Cerium oxide nanoparticles (nanoceria) are promising catalytic nanomaterials that are widely reported to modulate intracellular reactive oxygen species (ROS). In this study, nanoceria were synthesized by flame spray pyrolysis and functionalized with a cell-targeting ligand, folic acid (FA). The surface functionalization of nanoceria was stable, and FA enhanced the uptake of nanoceria via folate receptors.

Endocytosis of cerium oxide nanoparticles and modulation of reactive oxygen species in human ovarian and colon cancer cells.

Unlabelled: Cerium oxide nanoparticles (nanoceria) are widely reported to be cytocompatible and modulate intracellular reactive oxygen species (ROS) in a range of different cell types. In this study, nanoceria (d=7 and 94nm) synthesised by flame spray pyrolysis did not affect the proliferation of SKOV3 human ovarian and WiDr human colon cancer cell lines over a 72h treatment period. The cellular accumulation of nanoceria was uniform and increased up to 24h post-treatment before decreasing.

Hyaluronan coated cerium oxide nanoparticles modulate CD44 and reactive oxygen species expression in human fibroblasts.

Cerium oxide nanoparticles are being widely explored for cell therapies. In this study, nanoceria was functionalized with hyaluronan (HA) using the organosilane linker, 3-aminopropyltriethoxysilane. HA-nanoceria was found to be cytocompatible and to reduce intracellular reactive oxygen species in human fibroblasts.

Glycosaminoglycan functionalized nanoparticles exploit glycosaminoglycan functions.

Nanoparticles are being explored for a variety of applications including medical imaging, drug delivery, and biochemical detection. Surface functionalization of nanoparticles with glycosaminoglycans (GAGs) is an attractive strategy that is only starting to be investigated to improve their properties for biological and therapeutic applications. Herein, we describe a method to functionalize the surface of cerium oxide nanoparticles (nanoceria) with organosilane linkers, such as 3-(aminopropyl)triethoxysilane (APTES) and 3-(mercaptopropyl)trimethoxysilane (MPTMS), and GAGs, such as unfractionated and low molecular weight heparin.

Cellular uptake and reactive oxygen species modulation of cerium oxide nanoparticles in human monocyte cell line U937.

Cerium oxide nanoparticles (nanoceria) are promising materials for intracellular oxygen free radical scavenging providing a potential therapy for reactive oxygen species (ROS)-mediated inflammatory processes. In this study rhombohedral-shaped nanoceria were synthesized by flame spray pyrolysis with tuneable particle diameters between 3 and 94 nm by changing the liquid precursor flow rate. Monocytes and macrophages are major players in inflammatory processes as their production of ROS species has important downstream effects on cell signalling.