Biochemical and molecular characterization of novel keratinolytic protease from (KRLr1).

The keratin-degrading bacterium secretes a keratinase with potential industrial interest. Here, the Keratinase gene was intracellularly expressed in BL21(DE3) using pET-21b (+) vector. Phylogenetic tree analysis showed that KRLr1 is closely related to keratinase that belongs to the serine peptidase/subtilisin-like S8 family.

Prediction of B cell and T-helper cell epitopes candidates of bovine leukaemia virus (BLV) by in silico approach.

The bovine leukaemia virus (BLV) is a retrovirus responsible for enzootic bovine leukaemia (EBL) disease, the most common cattle disease leading to high annual economic losses to the cattle breeding industry. Virus monitoring among the sheep and cattle herds is usually done by vaccination. Inactivated virus vaccines can partially protect the livestock from viral challenge.

The effect of different levels of Fenugreek ( L.) powder and extract on performance, egg quality, blood parameters and immune responses of laying hens in second production cycle.

The present study was carried out to determine the effects of fenugreek powder (FP) and extract (FE) on performance, egg quality, blood parameters and immune responses of laying hens. One-hundred and fifty Leghorn laying hens were used in a completely randomized design with five treatments and five replicates for eight weeks. Treatments were various levels of FP and FE including zero (control; T1), 1.

RNA-Seq Bayesian Network Exploration of Immune System in Bovine.

Background: The stress is one of main factors effects on production system. Several factors (both genetic and environmental elements) regulate immune response to stress.

Objectives: In order to determine the major immune system regulatory genes underlying stress responses, a learning Bayesian network approach for those regulatory genes was applied to RNA-Seq data from a bovine leukocyte model system.

Reconstruction and Analysis of Cattle Metabolic Networks in Normal and Acidosis Rumen Tissue.

The objective of this study was to develop a system-level understanding of acidosis biology. Therefore, the genes expression differences between the normal and acidosis rumen epithelial tissues were first examined using the RNA-seq data in order to understand the molecular mechanisms involved in the disease and then their corresponding metabolic networks constructed. A number of 1074 genes, 978 isoforms, 1049 transcription start sites (TSS), 998 coding DNA sequence (CDS) and 2 promoters were identified being differentially expressed in the rumen tissue between the normal and acidosis samples ( < 0.

Genome-wide identification and characterization of novel long non-coding RNA in Ruminal tissue affected with sub-acute Ruminal acidosis from Holstein cattle.

Sub-acute ruminal acidosis is a type of metabolic disorder in which affected cattle show a considerable depression of rumen pH. This leads to a dramatic decline in productivity and consequent loss of income for many dairy farms. The objective of the present study is to identify and characterize novel long non-coding RNAs (lncRNAs) in Holstein cattle affected by sub-acute ruminal acidosis.

Investigation of rumen fermentation parameters and some blood metabolites of dromedary camels fed with C and C forages.

The aim of this experiment was to investigate rumen fermentation and some blood parameters of dromedary camels fed with C and C forage. Four fistulated dromedary adult camels were fed with diets as a changeover design, 30 days for each period. The diets included alfalfa hay + wheat straw (C forage) and atriplex+ suaeda + seidlitzia (C forage).

Effect of radiation on chemical composition and protein quality of cottonseed meal.

This study was conducted to compare the effects of electron beam (EB) and gamma ray (GR) irradiation treatments at doses of 10, 20 and 30 kGy on chemical composition, protein quality and protein digestibility of cottonseed meal (CSM). GR irradiation in all doses significantly decreased the crude fiber of samples compared to raw CSM. Free and total gossypol content of CSM was decreased significantly by utilizing both types of irradiation in a dose-dependent manner.

Phenotypic and genetic parameter estimates for reproductive traits in Zandi sheep.

This study reports on the phenotypic and genetic (co)variance components for reproductive traits in Zandi sheep, using between 1,859 and 2,588 records obtained from 577 ewes. The data were collected from the Khojir Breeding Station of Zandi sheep in Tehran, Iran from 1994 to 2008. The basic traits were litter size at birth (LSB), litter size at weaning (LSW), litter mean weight per lamb born (LMWLB), and litter mean weight per lamb weaned (LMWLW), and the composite traits were total litter weight at birth (TLWB) and total litter weight at weaning (TLWW).