Publications by authors named "Jaliya S Kumaratilake"
Article Synopsis
- Limb bud development in chicken embryos starts on day three due to FGF8 from the apical ectodermal ridge, leading to changes in the limb field mesoderm that cause the limbs to bulge out.
- Heintzelman et al. suggested that the bulging is due to higher liquid-like cohesivity in limb bud tissue compared to flank tissue, with experiments confirming that limb tissues are 1.5- to 2-fold more cohesive.
- Exposure to FGF8 can increase cohesivity in flank tissue, while the unique response of flank tissue to compression relies on an intact actin cytoskeleton, highlighting the importance of physical properties in tissue morphogenesis.
Background: Thermogenic brown adipose tissue has never been described in birds or other non-mammalian vertebrates. Brown adipocytes in mammals are distinguished from the more common white fat adipocytes by having numerous small lipid droplets rather than a single large one, elevated numbers of mitochondria, and mitochondrial expression of the nuclear gene UCP1, the uncoupler of oxidative phosphorylation responsible for non-shivering thermogenesis.
Results: We have identified in vitro inductive conditions in which mesenchymal cells isolated from the embryonic chicken limb bud differentiate into avian brown adipocyte-like cells (ABALCs) with the morphological and many of the biochemical properties of terminally differentiated brown adipocytes.