Publications by authors named "Jakub Zbigniew Kaczmarek"
Article Synopsis
- Understanding protein-protein interactions is vital for drug design, and several techniques like CryoEM, X-ray spectroscopy, and mass spectrometry are used to identify protein binding sites.
- The study proposes a new method for epitope mapping using Fenton chemistry, emphasizing the need for control experiments, particularly negative antibody controls, and employs TMT labeling for better quantification and fewer false positives.
- Findings from analyzing the SARS-CoV-2 Spike Protein RBD highlight significant structural changes when mixed with a negative control antibody, reinforcing the importance of control experiments and enhancing the reliability of the method in a typical mass spectrometry lab.
Tannerella forsythia is a gram-negative anaerobic bacterium that is associated with the development of destructive periodontal disease. T. forsythia secretes the metalloprotease-like enzyme karilysin.
View Article and Find Full Text PDFUrokinase-type plasminogen activator (uPA) is a trypsin-like serine protease that plays a vital role in extracellular conversion of inactive plasminogen into catalytically active plasmin. Activated plasmin facilitates the release of several proteolytic enzymes, which control processes like pericellular proteolysis and remodeling of ECM. uPA and the receptor uPAR, are overexpressed in a number of malignant tumours and uPA/uPAR play major roles in adhesion, migration, invasion and metastasis of cancer cells.
View Article and Find Full Text PDFPorphyromonas gingivalis is one of the major periodontitis-causing pathogens. P. gingivalis secretes a group of proteases termed gingipains, and in this study we have used the RgpB gingipain as a biomarker for P.
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