A Comparative Study on Immobilization of Fructosyltransferase in Biodegradable Polymers by Electrospinning.

Commercial application of biocatalysts depends on the efficiency of the immobilization method and residual enzyme activity. Electrospinning offers a simple and versatile route to immobilize enzymes in submicron-sized fibers and thus improved mass transfer characteristics. Performance of encapsulation of fructosyltransferase from Bacillus subtilis by emulsion, suspension, and coaxial electrospinning was compared.

Ion exchange resins as additives for efficient protein refolding by dialysis.

The most significant drawback of bacterial protein production involving inclusion bodies is the subsequent refolding into bioactive form. Implementation of refolding operations in large-scale applications often fails due to low yields and/or low product concentrations. This paper presents a simple method of integrated refolding by dialysis and matrix assisted refolding that combines advantages of both methods, high product concentrations and high refolding yields.

Effects of ionic strength on inclusion body refolding at high concentration.

For commercial applications refolding process must be fast, inexpensive and highly efficient. In the past many strategies for protein refolding were introduced. Still, simple refolding methods with high product concentrations are still rare.