Optimized amplitude modulation in femtosecond stimulated Raman microscopy.

In femtosecond stimulated Raman microscopy, two laser pulses (Raman pump and probe) interact at the focus of a scanning microscope. To retrieve the Raman signature of the sample, an amplitude modulation of the pump pulses is necessary. Here, different methods to achieve this modulation are presented and compared.

Broadband stimulated Raman microscopy with 0.1 ms pixel acquisition time.

Femtosecond stimulated Raman microscopy (FSRM) is a nonlinear technique for rapid broadband Raman imaging. It utilizes a few femtosecond probe pulse and a narrow bandwidth pump pulse. Using a fast (20 kHz) multi-channel detector, stimulated Raman spectra can be recorded with an acquisition time as short as 0.