Publications by authors named "Jakob M Schulze"
Over the past years, NGS has become a crucial workhorse for open-view pathogen diagnostics. Yet, long turnaround times result from using massively parallel high-throughput technologies as the analysis can only be performed after sequencing has finished. The interpretation of results can further be challenged by contaminations, clinically irrelevant sequences, and the sheer amount and complexity of the data.
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- HiLive is an innovative real-time read mapper that significantly reduces sample analysis time in clinical applications by mapping reads from Illumina sequencers while sequencing is still ongoing.
- It uses a k-mer based alignment method to continuously process the intermediate BCL files produced by sequencers, allowing for quicker results compared to traditional methods.
- Tests show that HiLive can provide final read alignments just minutes after sequencing ends, while standard file conversions typically take much longer, all while maintaining accuracy similar to other recent read mapping tools.
While initial phylogenetic analyses concluded to Guinea 2014 EBOV falling outside the Zaïre lineage (ZEBOV), a recent re-analysis of the same dataset by Dudas and Rambaut (2014) suggested that Guinea 2014 EBOV actually is ZEBOV. Under the same hypothesis as used by these authors (the molecular clock hypothesis), we reinforce their conclusion by providing a statistical assessment of the location of the root of the Zaïre lineage. Our analysis unambiguously supports Guinea 2014 EBOV as a member of the Zaïre lineage.
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