Vertical transmission of Zika virus in Culex quinquefasciatus Say and Aedes aegypti (L.) mosquitoes.

Several mosquito species have been described as vectors for the Zika virus (ZIKV), such as those in the Aedes, Anopheles, Mansonia and Culex genera. Our previous survey studies were found the ZIKV RNA positive in both male, female and larvae of Culex quinquefasciatus Say and Aedes aegypti (L.) mosquitoes collected from active ZIKV infected patients' homes in Thailand.

Vertical transmission of Indian Ocean Lineage of chikungunya virus in Aedes aegypti and Aedes albopictus mosquitoes.

Background: The re-emergence of chikungunya (CHIK) fever in Thailand has been caused by a novel lineage of chikungunya virus (CHIKV) termed the Indian Ocean Lineage (IOL). The Aedes albopictus mosquito is thought to be a primary vector of CHIK fever in Thailand, whereas Ae. aegypti acts as a secondary vector of the virus.

Morbidity Rate Prediction of Dengue Hemorrhagic Fever (DHF) Using the Support Vector Machine and the Aedes aegypti Infection Rate in Similar Climates and Geographical Areas.

Background: In the past few decades, several researchers have proposed highly accurate prediction models that have typically relied on climate parameters. However, climate factors can be unreliable and can lower the effectiveness of prediction when they are applied in locations where climate factors do not differ significantly. The purpose of this study was to improve a dengue surveillance system in areas with similar climate by exploiting the infection rate in the Aedes aegypti mosquito and using the support vector machine (SVM) technique for forecasting the dengue morbidity rate.

Comparative field efficacy of newly developed formulations of larvicides against Aedes aegypti (L.) (Diptera: Culicidae).

Aedes aegypti (L.) is known as vector of dengue and chikungunya fever. Larvicides are used to control this vector.

Insecticide resistance in bedbugs in Thailand and laboratory evaluation of insecticides for the control of Cimex hemipterus and Cimex lectularius (Hemiptera: Cimicidae).

Bedbugs are found in many countries around the world, and in some regions they are resistant to numerous insecticides. This study surveyed bedbugs in Thailand and determined their resistance to insecticides. The surveys were carried out in six provinces that attract large numbers of foreign tourists: Bangkok, Chonburi, Chiang Mai, Ubon Ratchathani, Phuket, and Krabi.

Intramolecular integration assay validates integrase phi C31 and R4 potential in a variety of insect cells.

Phage phi C31 and R4 integrases are site-specific and unidirectional serine recombinases. We have analyzed the ability of these integrases to mediate intramolecular integration between their attB and attP sites in 7 important insect cell lines as a means of predicting their relative mobility in the corresponding insect species. Both integrases exhibit significantly higher frequencies in Drosophila S2 cells than in the other insect cell lines examined, but do work well in all of the species tested.

Outbreak of chikungunya fever in Thailand and virus detection in field population of vector mosquitoes, Aedes aegypti (L.) and Aedes albopictus Skuse (Diptera: Culicidae).

We investigated chikungunya fever outbreak in the southern part of Thailand. Human plasma specimens obtained from suspected patients and adult wild-caught mosquitoes were detected for chikungunya virus employing reverse transcriptase-polymerase chain reaction technique. Chikungunya virus was detected in about half of the blood specimens whereas a range of 5.

Larvicidal efficacy of new formulations of temephos in non-woven sachets against larvae of Aedes aegypti (L.) (Diptera: Culicidae) in water-storage containers.

Three new formulations of temephos (LAVIFOS SG 1%, MOSQ SG 1% and AZAI-SS ZG 1%) were evaluated for larvicidal efficacy against larvae of Aedes aegypti (L.) in water-storage jars under field-simulated conditions. LAVIFOS SG 1% and MOSQ SG 1% are sand granule formulations, whereas AZAI-SS ZG 1% is zeolite granule formulation.

Field evaluation of novaluron, a chitin synthesis inhibitor larvicide, against mosquito larvae in polluted water in urban areas of Bangkok, Thailand.

Novaluron, an insect growth regulator, a benzoylphenyl urea insecticide, was evaluated in the field against the larvae of polluted-water mosquitoes. The study was carried out in highly polluted sites infested with populations of mosquito larvae, mostly Culex quinquefasciatus Say, in low-income communities in urban areas of Bangkok, Thailand. An EC10 formulation was premixed in water and applied by pressurized spray tank to plots ranging from 180 to 1,000 m2 at the rate of 0.

Cockroach surveys in the Northern region of Thailand and Guangxi Province of China.

Cockroach surveys were carried out in three provinces of the Northern region of Thailand: Chiang Mai, Chiang Rai, and Mae Hong Son, and three cities of Guangxi Province of the People's Republic of China: Nanning, Huangjiang, and Hechi. Sticky traps were used for cockroach sampling in these surveys. At least 30 houses in each province or city were randomly sampled.

Evaluation of attractants and egg-laying substrate preference for oviposition by Aedes albopictus (Diptera: Culicidae).

Evaluation of oviposition attractants and substrate preferences of Aedes albopictus was carried out under laboratory and field conditions. To obtain candidate oviposition substances we used a water rinse of 3 mollusk species: blood cockle (Anadara granosa), carpet shell (Paphia undulata), and sea mussel (Mytilus smaragdinus), and the giant tiger prawn (Penaeus monodon). The rinse water of carpet shell and giant tiger prawn showed higher attractiveness for ovipostion than the other candidate attractants.