Beyond Decay: Exploring the Age-associated Variations in and in Dental Caries.

Background: and play an important role in the etiopathogenesis and progression of dental caries (DC). Their quantification and identification may be helpful for epidemiological and early intervention measures.

Objectives: We conducted the study to evaluate the colony counts of and with the location of DC and correlate their prevalence with the age of the patient.

iDCNNPred: an interpretable deep learning model for virtual screening and identification of PI3Ka inhibitors against triple-negative breast cancer.

Triple-negative breast cancer (TNBC) lacks estrogen, progesterone, and HER2 expression, accounting for 15-20% of breast cancer cases. It is challenging due to low therapeutic response, heterogeneity, and aggressiveness. The PI3Ka isoform is a promising therapeutic target, often hyperactivated in TNBC, contributing to uncontrolled growth and cancer cell formation.

Innovative and polygonal trend analysis of temperature in agro climatic zones of India.

Understanding the long-term trends in temperature is crucial for assessing the impacts of climate change on agriculture, human and animal health, and natural habitats. An in-depth analysis of long-term spatio-temporal trends of monthly maximum and minimum temperatures along with Diurnal Temperature Range (DTR) from 1951 to 2022 in various Agro-Climatic Zones (ACZs) of India was performed using newly introduced Innovative Polygonal Trend Analysis (IPTA) and Innovative Trend Analysis (ITA) along with traditional Mann-Kendall (MK) or modified Mann-Kendall (m-MK) tests. The MK/m-MK and IPTA assessments detected significant trends in 44.

Electric vehicles in transition: Opportunities, challenges, and research agenda - A systematic literature review.

This study conducts a systematic literature review on electric vehicle (EV) adoption, mapping critical themes and presenting an integrated framework to advance understanding of EVs' role in sustainable transportation. Drawing on 917 Scopus-indexed articles and 23 stakeholder interviews, it explores economic, environmental, energy, and social (EEES) dynamics through the Theory-Context-Methodology (TCM) framework and causal loop diagrams. Findings reveal dominant theories, such as the Theory of Planned Behavior and Value-Belief Norm Theory, and underscore the importance of methodological approaches like regression analysis and structural equation modeling.

The effect of different clinical recycling methods on load deflection properties of super-elastic and thermal nickel-titanium orthodontic arch wires: A comparative assessment.

Introduction: Repeated clinical use of arch wires requires sterilization and may result in alteration of the properties of the wires as they get subjected to corrosion and cold working. Therefore, the present study aimed to assess the effects of different clinical recycling methods on the load-deflection properties of super-elastic and thermal nickel-titanium orthodontic arch wires.

Materials And Methods: A total of 50 0.

Identification of the novel HLA-B*49:01:01:22 allele in an Indian renal transplant donor by next generation sequencing.

HLA-B*49:01:01:22 differs from HLA-B*49:01:01:01 by a single nucleotide C->G change in intron 5 at gDNA 2451.

Deciphering the role of TLR3 polymorphisms in oral squamous cell carcinoma pathogenesis: A case-control study.

Background: Oral squamous cell carcinoma (OSCC) poses a significant global health burden, particularly prevalent in regions like India. Despite advancements in diagnostics, early detection of OSCC remains challenging, necessitating novel diagnostic modalities. Toll-like receptors (TLRs) and their polymorphisms have emerged as potential contributors to OSCC pathogenesis.

Enhancing monoclonal antibody stability during protein a chromatography using 2-methyl imidazolium dihydrogen phosphate.

This study investigates the impact of 2-methyl imidazolium dihydrogen phosphate (2-MIDHP) on monoclonal antibody (mAb) aggregation during the Protein A purification stage, at a low pH (pH 3.0), and the viral inactivation phase. Size-exclusion high-performance liquid chromatography (SE-HPLC) and dynamic light scattering (DLS) were used to assess the mAb aggregation.

Pulmonary mucormycosis eroding the chest wall: challenges in the management.

Pulmonary mucormycosis is a rare, life-threatening fungal infection usually seen in immunocompromised patients. Mortality in such patients is high due to underlying immunosuppression and poor general condition of the patients. Invasion of the adjacent structures is known but, to the best of our knowledge, pulmonary mucormycosis presenting with a full thickness chest wall erosion has not been reported.

Learning styles, approaches and academic performance of second and third-year medical students of a medical college of Kathmandu: a descriptive cross-sectional study.

Background: The research is based on a questionnaire to study the different learning styles among medical students, and their approach to intellectual development.

Methods: This is an observational, cross-sectional study conducted on 140 participants. Initially after the informed consent process, the second-year and third-year medical students were requested to respond to the questionnaire without consulting friends.

Prevalence of anti-HLA antibodies in COVID-19 convalescent plasma donors: an Indian experience.

Background: COVID-19 convalescent plasma is one of the experimental therapies used widely in moderately sick COVID-19 patients. However, there are a few risks involved in plasma transfusion; notably, transfusion-related acute lung injury (TRALI) caused by antibodies against human leukocyte antigens (HLA). This study was designed to assess the prevalence of anti-HLA antibodies in convalescent plasma donors using the single antigen bead method.

Correlates of diabetes mellitus and hypertension in India: Change as evidenced from NFHS- 4 and 5 during 2015-2021.

Both diabetes mellitus (DM) and hypertension (HTN) have been on the rise in recent decades all over the world more remarkably in developing countries like India. We intend to measure the prevalence of DM and HTN in the Indian population and to compare the trends and various correlates of these diseases in NFHS (National Family Health Survey)-4 and 5. Data of NFHS-4 and 5 were accessed from dhs program website.

The role of different physical function tests for the prediction of fracture risk in older women.

Background: Physical function is an important risk factor for fracture. Previous studies found that different physical tests (e.g.

Cryo-EM Structure of AAV2 Rep68 bound to integration site AAVS1: Insights into the mechanism of DNA melting.

The Rep68 protein from Adeno-Associated Virus (AAV) is a multifunctional SF3 helicase that performs most of the DNA transactions required for the viral life cycle. During AAV DNA replication, Rep68 assembles at the origin and catalyzes the DNA melting and nicking reactions during the hairpin rolling replication process to complete the second-strand synthesis of the AAV genome. Here, we report the Cryo-EM structures of Rep68 bound to double-stranded DNA (dsDNA) containing the sequence of the AAVS1 integration site in different nucleotide-bound states.

Clinical Evaluation of Coronally Advanced Flap with or without a Platelet-Rich Fibrin Membrane for the Root Coverage.

Aim: To evaluate "coronally advanced flap" with or without "a platelet-rich fibrin membrane for the root coverage."

Materials And Methods: All the clinical parameters were assessed at different time intervals (at baseline, 1, 3, and 6 months) in both experimental and control group. Following "clinical parameters" were recorded using "UNC-15" "Probe-Plaque Index (PI)" (Silness and Loe, 1964), "Gingival Index" (GI) (Loe and Silness, 1963), "Recession depth (RD)," "Recession width (RW)," "Clinical attachment level (CAL)," and "Width of keratinized gingiva (WKG)".

Recurrent mucinous cystic neoplasm of the mesentery in a young nullipara mimicking as ovarian carcinoma.

Inroduction: Mucinous cystic neoplasms are rare tumors. They may originate from either ovaries, pancreas, or other intra-abdominal sites, but rarely from the mesentery.

Case History: A 22-year-old nulliparaous woman, who had undergone laparascopic bilateral cystectomy for recurrent ovarian mass, presented with pain in abdomen, backache, and menstrual irregularities.

Augmenting K-Means Clustering With Qualitative Data to Discover the Engagement Patterns of Older Adults With Multimorbidity When Using Digital Health Technologies: Proof-of-Concept Trial.

Background: Multiple chronic conditions (multimorbidity) are becoming more prevalent among aging populations. Digital health technologies have the potential to assist in the self-management of multimorbidity, improving the awareness and monitoring of health and well-being, supporting a better understanding of the disease, and encouraging behavior change.

Objective: The aim of this study was to analyze how 60 older adults (mean age 74, SD 6.

Human CST complex restricts excessive PrimPol repriming upon UV induced replication stress by suppressing p21.

DNA replication stress, caused by various endogenous and exogenous agents, halt or stall DNA replication progression. Cells have developed diverse mechanisms to tolerate and overcome replication stress, enabling them to continue replication. One effective strategy to overcome stalled replication involves skipping the DNA lesion using a specialized polymerase known as PrimPol, which reinitiates DNA synthesis downstream of the damage.

Mucoepidermoid carcinoma of the lower lip: A case report and review of literature.

Mucoepidermoid carcinoma (MEC) is a rare salivary gland malignancy characterized by diverse cellular components. This case report presents a unique instance of low-grade MEC in a 28-year-old female, emphasizing the importance of accurate diagnosis and tailored management. The patient exhibited a painless, enlarging lower lip swelling over a year, with imaging suggestive of a fibrolipomatous lesion.

Outcomes of COVID-19 amongst patients with ongoing use of inhaled corticosteroids - a systematic review & meta-analysis.

Background: WHO quoted the numbers for the Coronavirus disease 2019 (COVID-19) pandemic as of August 2021 were 200 million cases with over 4 million deaths globally. COVID-19 is associated with several respiratory pathologies. Inhaled corticosteroids (ICS) are used to improve lung function by reducing inflammation, edema, mucus secretion, and inhibiting various cytokine activities.

CaMKK2 and CHK1 phosphorylate human STN1 in response to replication stress to protect stalled forks from aberrant resection.

Keeping replication fork stable is essential for safeguarding genome integrity; hence, its protection is highly regulated. The CTC1-STN1-TEN1 (CST) complex protects stalled forks from aberrant MRE11-mediated nascent strand DNA degradation (NSD). However, the activation mechanism for CST at forks is unknown.