A Valuable Source of Promising Extremophiles in Microbial Plastic Degradation.

Plastics have accumulated in open environments, such as oceans, rivers, and land, for centuries, but their effect has been of concern for only decades. Plastic pollution is a global challenge at the forefront of public awareness worldwide due to its negative effects on ecological systems, animals, human health, and national economies. Therefore, interest has increased regarding specific circular economies for the development of plastic production and the investigation of green technologies for plastic degradation after use on an appropriate timescale.

sp. nov., isolated from mountain soil.

Gram-stain-positive, aerobic, rod-shaped strains, YJM1 and YJM12S, were isolated from Maebong Mountain, Dogok-dong, Gangnam-gu, Seoul, Republic of Korea. Strains YJM1 and YJM12S exhibited growth at 5-35 °C (optimum, 20-30 °C) and pH 6-9 (optimum, pH 7) and in 0-4 % (w/v) NaCl. Strains YJM1 and YJM12S showed highest 16S rRNA gene sequence similarity to the following members of the genus : A33 (98.

Field application of bio-foam spray to reduce ammonia emission from ammonia-rich swine manure piles.

In this study, the first field-scale application of a bio-foam spray (a mixture of microbes and a surfactant) for the reduction of ammonia emitted from manure was investigated on six field swine manure piles. The objective of this study was to evaluate the odor suppression ability of bio-foam and odor degradation ability of odor-degrading bacteria loaded in the surfactant foam after covering manure piles. The size of field manure piles tested in this study ranged from 27 to 300 m.

Rice Husk-Cellulose-Based Agricultural Waste Enhances the Degradation of Synthetic Dyes Using Multiple Enzyme-Producing Extremophiles.

The brightly colored synthetic dyes used in the textile industry are discharged at high concentrations-for example, various azo dyes including Methylene Blue (MB) and Methyl Orange (MO)-which is a matter of global concern, as such dyes are harmful to humans and the environment. Microbial degradation is considered an efficient alternative for overcoming the disadvantages of conventional physical and chemical dye removal methods. In this study, we investigated the potential of multiple types of the enzyme-producing extremophilic bacteria FW2, isolated from food waste leachate, for the decolorization and bioremediation of artificial synthetic dyes.

sp. nov., isolated from forest soil in Pyeongchang, Republic of Korea.

Rod-shaped Gram-stain-negative, aerobic bacterial strains, designated PC14 and PC15, were isolated from a forest soil sample collected in Pyeongchang county, Gangwon-do, Republic of Korea. Strains PC14 and PC15 grew at 15-37 °C (optimum, 28-30 °C in tryptone soya agar and Mueller-Hinton agar), hydrolysed chitin and casein, and tolerated pH 8.5 and 2 % (w/v) NaCl.

Investigating Bio-Inspired Degradation of Toxic Dyes Using Potential Multi-Enzyme Producing Extremophiles.

Biological treatment methods overcome many of the drawbacks of physicochemical strategies and play a significant role in removing dye contamination for environmental sustainability. Numerous microorganisms have been investigated as promising dye-degrading candidates because of their high metabolic potential. However, few can be applied on a large scale because of the extremely harsh conditions in effluents polluted with multiple dyes, such as alkaline pH, high salinity/heavy metals/dye concentration, high temperature, and oxidative stress.

sp. nov., isolated from soil.

A white-pigmented, non-motile, Gram-stain-negative, rod-shaped bacterium, designated CYS-01, was obtained from soil sampled at Suwon, Gyeonggi-do, Republic of Korea. Cells were strictly aerobic, grew optimally at 28 °C. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain CYS-01 formed a lineage within the family and clustered with members of the genus .

Antioxidant and Antimicrobial Properties of Fruiting Body and Submerged Mycelium of Medicinal Mushroom Phellinus robiniae (Agaricomycetes).

This study was conducted to evaluate extraction yield, antioxidant content, antioxidant capacity and antibacterial activity of extracts obtained from submerged mycelium (ME) and fruiting body (FBE) of Phellinus robiniae NTH-PR1. The results showed that yields of ME and FBE reached 14.84 ± 0.

sp. nov. Isolated from Soil with Potential Antioxidant Activity.

A white-pigmented, non-motile, gram-negative, and rod-shaped bacterium, designated CYS-02, was isolated from soil sampled at Suwon, Gyeonggi-do, Republic of Korea. Cells were strictly aerobic, grew optimally at 20-28ºC and hydrolyzed Tween 40. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CYS-02 formed a lineage within the family and clustered as members of the genus .

Bacterial Biosorbents, an Efficient Heavy Metals Green Clean-Up Strategy: Prospects, Challenges, and Opportunities.

Rapid industrialization has led to the pollution of soil and water by various types of contaminants. Heavy metals (HMs) are considered the most reactive toxic contaminants, even at low concentrations, which cause health problems through accumulation in the food chain and water. Remediation using conventional methods, including physical and chemical techniques, is a costly treatment process and generates toxic by-products, which may negatively affect the surrounding environment.

Genome mining revealed polyhydroxybutyrate biosynthesis by Ramlibacter agri sp. nov., isolated from agriculture soil in Korea.

A white-colony-forming, facultative anaerobic, motile and Gram-stain-negative bacterium, designated G-1-2-2 was isolated from soil of agriculture field near Kyonggi University, Republic of Korea. Strain G-1-2-2 synthesized the polyhydroxybutyrate and could grow at 10-35 °C. The phylogenetic analysis based on 16S rRNA gene sequence showed that, strain G-1-2-2 formed a lineage within the family Comamonadaceae and clustered as a member of the genus Ramlibacter.

Coconut Mesocarp-Based Lignocellulosic Waste as a Substrate for Cellulase Production from High Promising Multienzyme-Producing FW2 without Pretreatments.

Facing the crucial issue of high cost in cellulase production from commercial celluloses, inexpensive lignocellulosic materials from agricultural wastes have been attractive. Therefore, several studies have focused on increasing the efficiency of cellulase production by potential microorganisms capable of secreting a high and diversified amount of enzymes using agricultural waste as valuable substrates. Especially, extremophilic bacteria play an important role in biorefinery due to their high value catalytic enzymes that are active even under harsh environmental conditions.

sp. nov., isolated from soil.

A yellow-pigmented, non-motile, Gram-stain-negative, rod-shaped bacterium, designated II4 was obtained from soil sampled at Seongnam, Gyeonggi-do, Republic of Korea. Cells were strictly aerobic, grew optimally at 20-28 °C and hydrolysed casein. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain II4 formed a lineage within the family and clustered as members of the genus .

Investigation of Lipolytic-Secreting Bacteria from an Artificially Polluted Soil Using a Modified Culture Method and Optimization of Their Lipase Production.

Compared to lipases from plants or animals, microbial lipases play a vital role in different industrial applications and biotechnological perspectives due to their high stability and cost-effectiveness. Therefore, numerous lipase producers have been investigated in a variety of environments in the presence of lipidic carbon and organic nitrogen sources. As a step in the development of cultivating the unculturable functional bacteria in this study, the forest soil collected from the surrounding plant roots was used to create an artificially contaminated environment for lipase-producing bacterial isolation.

Improvement of Hydrogen Production during Anaerobic Fermentation of Food Waste Leachate by Enriched Bacterial Culture Using Biochar as an Additive.

It has become urgent to develop cost-effective and clean technologies for the rapid and efficient treatment of food waste leachate, caused by the rapid accumulation of food waste volume. Moreover, to face the energy crisis, and to avoid dependence on non-renewable energy sources, the investigation of new sustainable and renewable energy sources from organic waste to energy conversion is an attractive option. Green energy biohydrogen production from food waste leachate, using a microbial pathway, is one of the most efficient technologies, due to its eco-friendly nature and high energy yield.

Description of antibiotic-producing novel bacteria sp. nov. and sp. nov.

Two white colony-forming, Gram-stain-negative, non-sporulating and motile bacteria, designated G-4-1-8 and RP-4-7, were isolated from forest soil and Arctic soil, respectively. Both strains showed antimicrobial activity against Gram-negative pathogens ( and ) and could grow at a pH range of pH 4.0-11.

Insights into the biodegradation of diesel oil and changes in bacterial communities in diesel-contaminated soil as a consequence of various soil amendments.

Soil amendment is a promising strategy to enhance biodegradation capacity of indigenous bacteria. To assess the consequences of various soil amendments before large-scale implementation, a microcosm study was employed to investigate the effects of nutrients (TN), surfactants (TS), oxidants (TO), biochar (TB), and zero-valent iron nanoparticles (nZVI; TNP) on diesel degradation, bacterial communities, and community-level physiological profiles (CLPPs) of legacy field contaminated soil. The results showed that the TN, TB, TNP, TS, and TO, reduced 75.

sp. nov., a Nitrogen-Fixing Bacterium Isolated From Forest Soil by Using Optimized Culture Method.

A bacterial strain, designated strain G-1-1-14, was isolated from Kyonggi University forest soil during a study of previously uncultured bacterium. The cells of strain G-1-1-14 were motile by means of peritrichous flagella, Gram-stain-negative, rod-shaped, and able to grow autotrophically with hydrogen and fix nitrogen. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain G-1-1-14 belonged to the genus .

Cold-shock gene cspC in the genome of Massilia polaris sp. nov. revealed cold-adaptation.

A straw coloured, motile and Gram-stain-negative bacterium, designated RP-1-19 was isolated from soil of Arctic station, Svalbard, Norway. Based on the phylogenetic analysis of its 16S rRNA gene sequence, strain RP-1-19 formed a lineage within the family Oxalobacteraceae and clustered together within the genus Massilia. The closest members were M.

Genome insight and description of antibiotic producing Massilia antibiotica sp. nov., isolated from oil-contaminated soil.

An ivory-coloured, motile, Gram-stain-negative bacterium, designated TW-1 was isolated from oil-contaminated experimental soil in Kyonggi University. The phylogenetic analysis based on 16S rRNA gene sequence revealed, strain TW-1 formed a lineage within the family Oxalobacteraceae and clustered as members of the genus Massilia. The closest members were M.

Description of sp. nov., a cold-adapted bacterium isolated from Arctic soil.

A yellow-coloured, Gram-stain-negative, non-sporulating, psychrotolerant and motile bacterium, designated AR-3-1, was isolated from the Arctic soil of Cambridge Bay, Nunavut, Canada. Strain AR-3-1 could grow at 4-32 °C and pH 5.0- 11.

sp. nov., isolated from forest soil.

A Gram-stain-positive, aerobic and rod-shaped bacterial strain, designated JH1-1, was isolated from a forest soil sample collected in Suwon, Gyeonggi-do, Republic of Korea. Strain JH1-1 could grow at 10-35 °C (optimum, 28-30 °C), pH 4.5-8.

Effect of consortium bioaugmentation and biostimulation on remediation efficiency and bacterial diversity of diesel-contaminated aged soil.

This study aimed to evaluate the effects of consortium bioaugmentation (CB) and various biostimulation options on the remediation efficiency and bacterial diversity of diesel-contaminated aged soil. The bacterial consortium was prepared using strains D-46, D-99, D134-1, MSM-2-10-13, and Oil-4, isolated from oil-contaminated soil. The effects of CB and biostimulation were evaluated in various soil microcosms: CT (water), T1 (CB only), T2 (CB + NHNO and KHPO nutrients), T3 (CB + activated charcoal, AC), T4 (CB + nutrients + AC), T5 (AC + water), T6 (CB + nutrients + zero-valent iron nanoparticles, nZVI), T7 (CB + nutrients + AC + nZVI), T8 (CB + activated peroxidase, oxidant), T9 (AC + nZVI), and T10 (CB + nZVI + AC + oxidant).