Publications by authors named "Jaishree Sijapati"
Article Synopsis
- This study focused on isolating and characterizing a thermophilic bacterium from Himalayan soil that produces cellulase, with the PANG L strain being the most effective identified.
- The optimal conditions for cellulase production were found to be at 45°C and pH 6.5 using carboxymethyl cellulose and yeast extract as nutrient sources, achieving partial purification of the enzyme.
- The cellulase enzyme demonstrated high activity at 60°C and pH 5, exhibited stability in acidic conditions, and showed potential for industrial applications due to its enhanced activity with certain metal ions.
This study is aimed at isolating and identifying a thermophilic cellulolytic bacterium from hot spring water and characterizing thermostable cellulase produced by the isolate. Enrichment and culture of water sample was used for isolation of bacterial strains and an isolate with highest cellulase activity was chosen for the production, partial purification, and biochemical characterization of the enzyme. Different staining techniques, enzymatic tests, and 16s ribosomal DNA (16s rDNA) gene sequencing were used for the identification of the isolate.
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