Publications by authors named "Jairo Rodriguez Chalarca"
Bioassays were conducted under controlled conditions to determine the response of (J. E. Smith) larvae fed with corn materials expressing (Bt) insecticidal endotoxins: (1) VT Double Pro (VT2P) expressing Cry1A.
View Article and Find Full Text PDFArticle Synopsis
- * Traditional biological control methods are becoming less effective, prompting the exploration of new management strategies such as transgenic plants that utilize insecticidal proteins from Bacillus thuringiensis (Bt).
- * A study successfully developed a bioassay method using fresh corn tissue to test the effectiveness of the Cry1Ac protein against various Diatraea species, revealing over 90% mortality and growth inhibition in test subjects.
An optimized high-quality DNA isolation protocol was developed using body segment tissue from the Fall Armyworm (Spodoptera frugiperda), that will allow documenting genetic variability based on biotypes, facilitating studies on the appearance, distribution and population dynamics of the fall armyworm at the molecular level. The resulting protocol is an easy-to-use, timesaving method that can rapidly achieve high quality, high-yielding total genomic DNA, using chemicals and everyday consumables available in a molecular laboratory. This new method of DNA extraction avoids the contamination of polysaccharides, salts, phenols, proteins and other cellular by-products that can interfere with subsequent reactions.
View Article and Find Full Text PDF