Daily methionine requirements of healthy Indian men, measured by a 24-h indicator amino acid oxidation and balance technique.

Background: The 1985 FAO/WHO/UNU upper requirement for the sulfur-containing amino acids in healthy adults, which was set at 13 mg . kg(-)(1) . d(-)(1), is based on nitrogen balance studies in Western subjects.

Lysine requirements of chronically undernourished adult Indian men, measured by a 24-h indicator amino acid oxidation and balance technique.

Background: In earlier studies with well-nourished subjects that used a 24-h indicator amino acid oxidation or balance approach, we concluded that the 1985 FAO/WHO/UNU requirement for lysine (12 mg x kg(-1) x d(-1)) was inadequate for healthy South Asian subjects and proposed a tentative requirement of 30 mg x kg(-1) x d(-1).

Objective: We assessed whether chronic undernutrition, with low habitual dietary protein and lysine intakes, leads to changed lysine requirements.

Design: Twenty-seven otherwise clinically healthy, chronically undernourished Indian men were studied during 2 randomly assigned 7-d diet periods supplying 12 and 30, 18 and 36, or 24 and 42 mg lysine x kg(-1) x d(-1), based on an L-amino acid diet.

Threonine requirements of healthy Indian men, measured by a 24-h indicator amino acid oxidation and balance technique.

Background: We previously questioned the validity of the 1985 FAO/WHO/UNU upper requirement value for threonine (7 mg x kg(-1) x d(-1)) and proposed a tentative mean requirement of 15 mg x kg(-1) x d(-1).

Objective: In this study we used a 24-h indicator amino acid oxidation and balance technique, with [1-(13)C]leucine as the indicator amino acid, to assess threonine adequacy at 6 test intakes (7, 11, 15, 19, 22, and 27 mg x kg(-1) x d(-1)) with a 6-d dietary adaptation phase in healthy, well-nourished Indian men.

Design: Sixteen men were randomly allocated to 3 of 6 test intakes and were studied after 6 d of adaptation to the experimental diets.