Protective effect of dietary long-chain n-3 polyunsaturated fatty acids on bone loss in gonad-intact middle-aged male rats.

This study evaluated the effect of a fat blend containing long-chain (LC) n-3 PUFA on bone mineral density (BMD) and bone metabolism in gonad-intact middle-aged male rats (12 months old, n 28). Seven rats were killed on day 0 of dietary intervention to determine the baseline BMD. The remaining rats (seven per group) were fed a diet with one of the following dietary lipid treatments (g/kg diet): 167 g safflower oil + 33 g menhaden oil (N6 + N3 diet, control), 200 g safflower oil (N6 diet, almost devoid of LC n-3 PUFA), or 190 g menhaden oil + 10 g corn oil (N3 diet, rich in LC n-3 PUFA) for 20 weeks.

The effect of local hematoma blocks on early fracture healing.

To test the effects of lidocaine and bupivacaine on early fracture healing, the tensile strength of five groups of femora from normal rats 14 and 35 days after production of a closed diaphyseal fracture were compared. Data indicate that, in experimental animals injected with local anesthetics at the fracture site, no measurable difference is noted in callus composition, tensile strength, or histological appearance during the early phases of fracture repair prior to callus remodeling.