In vitro anti-biofilm efficacy of therapeutic low dose 265 nm UVC.

Purpose: Preclinical studies have confirmed the safety and efficacy of narrowband low-intensity ultraviolet C light (UVC) in managing bacterial corneal infection. To further consolidate these findings, the present study aimed to explore in vitro anti-biofilm efficacy of low-intensity UVC light for its potential use in biofilm-related infections.

Methods: Pseudomonas aeruginosa biofilm was grown in chamber well slides for 48 h and exposed to one of the following challenges: UVC (265 nm wavelength, intensity 1.

Correction: Optimal dose of lactoferrin reduces the resilience of in vitro Staphylococcus aureus colonies.

[This corrects the article DOI: 10.1371/journal.pone.

Optimal dose of lactoferrin reduces the resilience of in vitro Staphylococcus aureus colonies.

The rise in antibiotic resistance has stimulated research into adjuvants that can improve the efficacy of broad-spectrum antibiotics. Lactoferrin is a candidate adjuvant; it is a multifunctional iron-binding protein with antimicrobial properties. It is known to show dose-dependent antimicrobial activity against Staphylococcus aureus through iron sequestration and repression of β-lactamase expression.

Simplifying the Process of Going From Cells to Tissues Using Statistical Mechanics.

The value of digital twins for prototyping controllers or interventions in a sandbox environment are well-established in engineering and physics. However, this is challenging for biophysics trying to seamlessly compose models of multiple spatial and temporal scale behavior into the digital twin. Two challenges stand out as constraining progress: (i) ensuring physical consistency of conservation laws across composite models and (ii) drawing useful and timely clinical and scientific information from conceptually and computationally complex models.

Our natural "makeup" reveals more than it hides: Modeling the skin and its microbiome.

Skin is our primary interface with the environment. A structurally and functionally complex organ that hosts a dynamic ecosystem of microbes, and synthesizes many compounds that affect our well-being and psychosocial interactions. It is a natural platform of signal exchange between internal organs, skin resident microbes, and the environment.

Inferring intra-cellular mechanics using geometric metamorphosis: A preliminary study.

Mechanotransduction plays an important role in sub-cellular processes and is an active area of research. Determining the forces/strains that the intra-cellular structures experience is vital for developing quantitative models of cellular behavior. Established techniques such as traction force microscopy, digital image correlation etc.

Using CellML with OpenCMISS to Simulate Multi-Scale Physiology.

OpenCMISS is an open-source modeling environment aimed, in particular, at the solution of bioengineering problems. OpenCMISS consists of two main parts: a computational library (OpenCMISS-Iron) and a field manipulation and visualization library (OpenCMISS-Zinc). OpenCMISS is designed for the solution of coupled multi-scale, multi-physics problems in a general-purpose parallel environment.

ICMA: an integrated cardiac modeling and analysis platform.

Unlabelled: ICMA, a software framework to create 3D finite element models of the left ventricle from cardiac ultrasound or magnetic resonance imaging (MRI) data, has been made available as an open-source code. The framework is hardware vendor independent and uses speckle tracking (endocardial border detection) on ultrasound (MRI) imaging data in the form of DICOM. Standard American Heart Association segment-based strain analysis can be performed using a browser-based interface.

Open access integrated therapeutic and diagnostic platforms for personalized cardiovascular medicine.

It is undeniable that the increasing costs in healthcare are a concern. Although technological advancements have been made in healthcare systems, the return on investment made by governments and payers has been poor. The current model of care is unsustainable and is due for an upgrade.

A mean-field model of ventricular muscle tissue.

A theoretical model of the cross-linking topology of ventricular muscle tissue is developed. Using parameter estimation the terms of the theoretical model are estimated for normal and pathological conditions. The model represents the anisotropic structure of the tissue, reproduces published experimental data and characterizes the role of different tissue components in the observed macroscopic behavior.

Efficient solutions of cardiac membrane models using novel unsupervised clustering algorithm.

We present a method to efficiently solve cardiac membrane models using a novel unsupervised clustering algorithm. The unsupervised clustering algorithm was designed to handle repeated clustering of multidimensional objects with rapidly changing properties. A Modified Trie datastructure that allowed efficient search, scalable and distributed assembly of the result was designed.