Optimization of PCR-RFLP Directly from the Skin and Nails in Cases of Dermatophytosis, Targeting the ITS and the 18S Ribosomal DNA Regions.

Purpose: A pan fungal primer targeting the Internal Transcribed Spacer (ITS) region and optimization of PCR-RFLP using a dermatophyte specific primer targeted the 18S ribosomal DNA (rDNA) region were performed for the identification of dermatophyte species and strains directly from clinical specimens.

Materials And Methods: One hundred and thirty eight specimens (129 skin scrapings and 9 nail clippings) from clinically suspected cases of dermatophytosis were collected and subjected to direct microscopy and culture. Among them, 66 skin scrapings and 3 nail clippings were processed for genotyping by PCR-RFLP analysis using the Mva I, Hae III and the Dde I restriction enzymes.

Assessment of aerobic bacterial contamination of computer keyboards in a tropical setting.

Background: Computers are widely used in healthcare for improved and effective care. Previous published reports have shown microorganisms colonising computer keyboards in some clinical areas.

Objectives: This study was undertaken to measure, compare and characterize the aerobic microorganisms in computer keyboards of hospital and non-hospital settings.