Publications by authors named "Jagadeesh Babu Sreemanthula"
Article Synopsis
- * Analysis involved 2,431 cases and 1,265 healthy controls, revealing that individuals with the MTHFR variant had a significantly higher risk for conditions like respiratory distress, recurrent pregnancy loss, and intellectual disabilities.
- * The MTHFR TT-genotype specifically indicated a high risk of abnormal phenotypes, emphasizing the importance of this genetic variant in neurodevelopmental and other health-related outcomes.
Epithelial cell adhesion molecule (EpCAM), a cancer stem cell (CSC) marker is over expressed in epithelial cancers and in retinoblastoma (RB). We fabricated an EpCAM targeting aptamer-siRNA chimera and investigated its anti-tumor property and EpCAM intracellular domain (EpICD) mediated signaling in epithelial cancer. The anti-tumor efficacy of EpCAM aptamer-siEpCAM chimera (EpApt-siEp) was evaluated by qPCR, northern and Western blotting in WERI-Rb1- RB cell line, primary RB tumor cells and in MCF7- breast cancer cell line.
View Article and Find Full Text PDFFor the first time, a novel EpCAM aptamer (SYL3C)-DIBO-AF594 fluorescent conjugate was synthesised by bioorthogonal chemistry utilizing a strain promoted alkyne-azide cycloaddition (copper free click) reaction (SPAAC). The ligation efficiency of SPAAC was improved by freeze-thaw cycles. The obtained conjugate showed target specific binding and aided in the imaging of various EpCAM positive cancer cell lines like MCF7, MDAMB453, Weri-RB1 and PC3.
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