Increase CO recycling of containing CBB genes by enhancing solubility of multiple expressed proteins from an operon through temperature reduction.

In a previous study, we successfully engineered capable of endogenous CO recycling through the heterologous expression of the Calvin-Benson Bassham genes. Establishing an efficient gene expression environment for recombinant strains is crucial, on par with the importance of metabolic engineering design. Therefore, the primary objective of this study was to further mitigate greenhouse gas emissions by investigating the effects of culture temperature on the formation of inclusion bodies (IB) and CO fixation activity in the engineered bacterial strain.

Detection of triclosan using 2-domain hemoglobin promoter of Daphnia magna.

Triclosan has been widely used as an antimicrobial agent. However, triclosan was found to cause toxicity, including muscle contraction disturbances, carcinogenesis, and endocrine disorders. In addition, it was found to affect central nervous system function adversely and even have ototoxic effects.

Detection of Salmonella dublin using the vitellogenin 2 promoter of Daphnia magna.

Salmonella is a well-known bacterium that causes waterborne diseases in humans and primates. The need for test models to detect such pathogens and study the responses of such organisms to induced toxic environments is vital. Daphnia magna has been ubiquitously used in aquatic life monitoring for decades because of outstanding properties, such as facile cultivation, short lifespan, and high reproductive capacity.

Evaluation of Growth and Utilization Potential of Rhodobacter sphaeroides in Reused Medium.

Rhodobacter sphaeroides is a metabolically versatile purple non-sulfur bacteria that can produce valuable substances. As the low-cost and high-efficiency production of valuable substances is attracting attention, the reuse of the medium is emerging as a promising strategy. Therefore, in this study, the growth of R.

Optimized Rhodobacter sphaeroides for the Production of Antioxidants and the Pigments with Antioxidant Activity.

The photosynthetic bacterium, Rhodobacter sphaeroides, is a bacterium that can grow in a variety of environments and produces substances with antioxidant effects. In this study, we investigated the culture conditions to increase the production of antioxidants in R. sphaeroides, which can grow under both aerobic and anaerobic conditions.

Isoprene production by Rhodobacter sphaeroides and its antimicrobial activity.

Extensive studies on the antimicrobial activity of terpene-based substances, which are the main components of essential oils, are continuously underway. And some hydrocarbons constituting antimicrobial substances have been reported to exhibit the antimicrobial activity. This study confirmed the production of isoprene, the most basic constituent hydrocarbon of terpene, by Rhodobacter sphaeroides, and investigated the antimicrobial activity of isoprene and its mechanism.

Evaluation of muscle damage using ultrasound imaging.

[Purpose] This study aimed to quantitatively analyze characteristics of and changes in internal muscle structure according to the time of delayed onset muscle soreness (DOMS) using ultrasound imaging, thereby presenting clinical evidential data for evaluation of muscle damage. [Subjects] We recruited 38 male subjects. [Methods] Ultrasound images of the medial gastrocnemius muscle prior to induction of DOMS and immediately after, 24 hours after, 48 hours after, and 72 hours after induction of DOMS were obtained, and the thickness and pennation angle of the muscle were measured.

Estimation of mercury speciation in soil standard reference materials with different extraction methods by ion chromatography coupled with ICP-MS.

Analytical methods for the speciation of mercury, based on microwave extraction and sonication extraction, have been tested to determine the inorganic mercury and methyl mercury contents in two standard soil reference materials: SRM 2710 Montana Soil and BCR 580 estuarine sediment. Prior to applying the speciation extraction methods, the mineral compositions were analyzed via XRD analysis, with SRM 2710 shown to be composed mostly of aluminum silicate minerals, while carbonate minerals were the major constituent in BCR 580. Two extraction methods, microwave and sonication, were tested for the analysis and recovery efficiency of total mercury.

Erectile potentials of a new phosphodiesterase type 5 inhibitor, DA-8159, in diet-induced obese rats.

Aim: To examine the changes in the erectile function in diet-induced obese rats and investigate the oral efficacy of DA-8159, a new phosphodiesterase type 5 (PDE5) inhibitor, on penile erection in obese rats.

Methods: The rats were fed a high-energy diet for 12 weeks and divided into three groups: an obesity-resistant (OR) control group, an obesity-prone (OP) control group, and an OP-DA-8159 treatment (DA-8159) group. The electrostimulation-induced erectile responses were measured in all groups.