Publications by authors named "Jaewon Huh"

Opioid-sparing anesthesia (OSA) using dexmedetomidine has gained attention as an alternative to opioid-based anesthesia (OBA) due to its potential to reduce opioid consumption and the associated side effects. This study aimed to investigate the effect of dexmedetomidine-based OSA on postoperative pain intensity, opioid consumption, and recovery outcomes in patients undergoing a minimally invasive repair of pectus excavatum. Eighty-four patients undergoing a minimally invasive repair of pectus excavatum were randomized to either the OSA group, receiving dexmedetomidine, or the OBA group, receiving remifentanil.

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Lung cancer remains a leading cause of cancer-related mortality worldwide. Although surgical treatment is a primary approach, residual cancer cells and surgery-induced pathophysiological changes may promote cancer recurrence and metastasis. Anesthetic agents and techniques have recently been shown to potentially impact these processes by modulating surgical stress responses, immune function, inflammatory pathways, and the tumor microenvironment.

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: Postreperfusion syndrome (PRS) is a significant challenge in liver transplantation (LT), leading to severe circulatory and metabolic complications. Ischemic preconditioning (IPC), including remote IPC (RIPC), can mitigate ischemia-reperfusion injury, although its efficacy in LT remains unclear. This study evaluated the impact of paired RIPC, involving the application of RIPC to both the recipient and the living donor, on the incidence of PRS and the need for rescue epinephrine during living-donor LT (LDLT).

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: This study explored the effect of paired remote ischemic preconditioning (RIPC), involving both recipients and living donors, on cardiovascular stress in recipients after living-donor kidney transplantation (LDKT). The analysis included an assessment of the impact on cardiovascular biomarkers and post-transplant cardiovascular clinical events. : A retrospective observational cohort study of 520 adult LDKT patients was conducted, employing propensity score matching (PSM) to analyze perioperative factors.

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With growing concerns about opioid-related risks, efforts to reduce opioid use throughout the perioperative period have increased. This study aimed to compare postoperative recovery outcomes between opioid-free anesthesia (OFA) and opioid-sparing anesthesia (OSA) under a multimodal analgesic protocol in video-assisted thoracoscopic surgery (VATS). A retrospective cohort study was conducted on 196 patients undergoing VATS from August 2019 to December 2021.

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Perioperative dysglycemia is associated with negative surgical outcomes, including increased risk of infections and longer hospital stays. Continuous glucose monitoring (CGM) provides real-time glucose data, potentially improving glycemic control during surgery. However, the performance of CGM in the intraoperative environment has not been well established.

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Opioids effectively manage perioperative pain but have numerous adverse effects. Opioid-free anesthesia (OFA) eliminates intraoperative opioid use; however, evidence for its use in video-assisted thoracoscopic surgery (VATS) is limited. This study assessed the effect of OFA using ketamine in VATS patients compared to opioid-sparing anesthesia (OSA).

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Living donor kidney transplantation (LDKT) is a crucial treatment for end-stage renal disease, with pre-emptive LDKT (transplantation before dialysis initiation) offering significant benefits in graft function and patient survival. The selection of a vasopressor during LDKT, particularly between norepinephrine and dopamine, and its impact on renal arterial hemodynamics measured using the renal arterial resistive index (RARI) is poorly understood. This retrospective observational cohort study enrolled 347 eligible pre-emptive LDKT recipients from the Seoul St.

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: Preoperative fasting guidelines traditionally aim to reduce pulmonary aspiration risk. However, concerns over the adverse effects of prolonged fasting have led to exploring alternatives. This study aimed to investigate the impact of preoperative clear liquid intake on postoperative outcomes in children undergoing minimally invasive repair of pectus excavatum (MIRPE).

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: Immediate breast reconstruction surgery (BRS) often leads to significant postoperative pain, necessitating effective analgesia. This study aimed to compare the analgesic efficacy of patient-controlled analgesia (PCA) containing nefopam with that of PCA containing opioids alone in patients undergoing BRS. : A prospective, double-blind, randomized controlled trial was conducted on 120 patients undergoing immediate BRS after mastectomy.

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Article Synopsis
  • Aging research is still largely unexplored, prompting an investigation into the immune response characteristics of healthy male cynomolgus macaques across different age groups.* -
  • The study identified three distinct aging patterns in gene expression related to immune responses: increased expression linked to innate immunity causing chronic inflammation, and decreased expression linked to adaptive immunity affecting antibody diversity.* -
  • Findings suggest that the gene expression patterns in macaques reflect aging processes and correlate with human disease states, potentially aiding in future predictions of human health issues.*
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The CRISPR-Cas nickase system for genome editing has attracted considerable attention owing to its safety, efficiency, and versatility. Although alternative effectors to Cas9 have the potential to expand the scope of genome editing, their application has not been optimized. Herein, we used an enhanced CRISPR-Cas12a nickase system to induce mutations by targeting genes in a human-derived cell line.

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Children with pectus excavatum are treated with surgical repair in a procedure known as minimally invasive repair of pectus excavatum (MIRPE). MIRPE causes considerable postoperative pain, resulting in the administration of a substantial dose of opioids. This study aimed to identify perioperative factors that influence the requirement for opioids in children undergoing MIRPE.

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Imaging two or more fluorescent biosensors in the same living cell can reveal the spatiotemporal coordination of protein activities. However, using multiple Förster resonance energy transfer (FRET) biosensors together is challenging due to toxicity and the need for orthogonal fluorophores. Here we generate a biosensor component that binds selectively to the activated conformation of three different proteins.

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Background: Melatonin is a multifunctional hormone synthesized in the pineal gland and peripheral reproductive tissues that regulates many biological processes. There is increasing evidence for a role of melatonin in oocyte maturation and embryonic development in various mammals. However, no study has reported evidence for the existence of melatonergic system, such as melatonin synthesis enzymes, melatonin membrane receptors, or melatonin binding sites in non-human primate cumulus-oocyte complexes (COCs).

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Biogenic amines are cellular components produced by the decarboxylation of amino acids; however, excessive biogenic amine production causes adverse health problems. The relationship between hepatic damage and biogenic amine levels in nonalcoholic fatty liver disease (NAFLD) remains unclear. In this study, mice were fed a high-fat diet (HFD) for 10 weeks to induce obesity, presenting early-stage of NAFLD.

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Article Synopsis
  • * It investigates how the uneven distribution of MPTP due to varying cerebral blood flow affects damage in specific brain regions, particularly the striatum, in three monkeys.
  • * Findings indicate that differences in blood flow velocity and anatomical structure of the circle of Willis can lead to asymmetrical brain damage, suggesting these factors should be considered for better Parkinson's models.
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Article Synopsis
  • The CRISPR-Cas12a system utilizes a Cas12a protein and a guide RNA to specifically edit genes by targeting DNA sequences.
  • Recent improvements in this system involved engineering the protein and the guide RNA to enhance both efficiency and specificity for better gene editing results.
  • The study found that using an enhanced Cas12a system with a chimeric DNA-RNA guide significantly increased gene-editing efficiency and accuracy, potentially advancing applications like human gene therapy.
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  • MicroRNAs (miRNAs), like hsa-miR-422a (derived from SINE), play a key role in regulating gene expression at the post-transcriptional level by targeting complementary mRNA sequences.
  • The study identified AT-Rich Interaction Domain 5 B as a target gene for hsa-miR-422a and used a dual luciferase assay in HepG2 cells to confirm the miRNA's impact on gene expression.
  • Results showed that hsa-miR-422a enhances gene expression by interacting with transcription factors, particularly NF-E2, indicating its role as a super enhancer miRNA.
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Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase. In this study, we develop a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse transcriptase to the Francisella novicida Cas9, which is a CRISPR-Cas9 ortholog. Using FnCas9(H969A) nickase, the targeting limitation of existing Streptococcus pyogenes Cas9 nickase [SpCas9(H840A)]-based prime editing is dramatically extended, and accurate prime editing is induced specifically for the target genes in human cell lines.

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Purpose: Postoperative delirium (POD) is a common but serious complication after cardiac surgery and is associated with various short- and long-term outcomes. In this study, we investigated the effects of intraoperative glycemic variability (GV) and other glycemic variables on POD after cardiac surgery.

Patients And Methods: A retrospective single-center cohort analysis was conducted using data from electronic medical record from 2018 to 2020.

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Background: Pulmonary artery catheter insertion is a routine practice in high-risk patients undergoing cardiac surgery. However, pulmonary artery catheter insertion is associated with numerous complications that can be devastating to the patient. Incorrect placement is an overlooked complication with few case reports to date.

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Background: Obesity due to an excessive intake of nutrient disturbs the hypothalamus-mediated energy metabolism subsequently develops metabolic disorders. In this study, we investigated the effect of pine needle extract (PNE) on the hypothalamic proopiomelanocortin (POMC) neurons involved in the regulation of energy balance via melanocortin system and fat tissue metabolism.

Methods: We performed electrophysiological and immunohistochemical analyses to determine the effect of PNE on POMC neurons.

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Article Synopsis
  • Excess glutamate is vital for the neuronal system, but too much can lead to excitotoxicity, causing damage to neuronal cells.
  • This study investigates whether mitochondrial or cytosolic Peroxiredoxin 5 (Prx5) offers more neuroprotection against glutamate toxicity, finding that mitochondrial Prx5 is more effective.
  • The research highlights the roles of reactive oxygen species (ROS) in both cytosolic and mitochondrial contexts, suggesting these factors could be targeted in therapies to prevent neurodegenerative diseases.
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