This study introduces a novel deep learning-based technique for predicting pressure distribution images, aimed at application in image-based approximate optimal design. The proposed approach integrates both unsupervised and supervised learning paradigms, employing autoencoders (AE) for the unsupervised component and fully connected neural networks (FNN) for the supervised component. A surrogate model based on 2D image data was developed, enabling a comparative analysis of three distinct methods: the conventional AE, the convolutional autoencoder (CAE), and a hybrid CAE, which combines the CAE with a conventional AE.
View Article and Find Full Text PDFThe study aimed to evaluate the synergistic interaction of organic acids (OAAs) or lemon extract (LE) plus mild heat (MH; 55 °C) against O157:H7, serovar Typhimurium, and inoculated in beetroot and watermelon juices. A mixed culture cocktail of . O157:H7, .
View Article and Find Full Text PDFFood processing industries commonly employ organic acids (OAAs) to determine bacterial contamination in acidified and fermented foods. OAAs are believed to possess potent antimicrobial properties by permeating cell membranes, altering proton and anion concentrations in the cytoplasm due to their lipophilic undissociated forms. The bacteriostatic or bactericidal effects of OAAs are influenced by various factors including microbial physiology, environmental pH, and acid dissociation ratios.
View Article and Find Full Text PDFUnlabelled: This study analyzed the virulence, growth characteristics, and cytotoxicity of strains isolated from fresh produce, including romaine lettuce, sesame leaf, tomato, and cucumber grown by different methods. Polymerase chain reaction (PCR) was used to assess the toxigenic potential, and the cytotoxicity of was estimated using cell-free supernatant in HEp-2 cells. The study found that was the predominant diarrheal enterotoxin in the 59 isolated strains, followed by and .
View Article and Find Full Text PDFLoud footsteps from upstairs cause disturbance to downstairs neighbours in multi-story residential buildings. In this experiment, we examined how participants walk when asked to walk quietly and evaluated the efficiency of their quiet walking patterns. Changes in vertical impact loading rates during the early stance phase, walking speed, and lower limb muscle activity when asked to walk quietly were evaluated from twenty-six young participants.
View Article and Find Full Text PDFThis study aims to investigate the resistance of E. coli O157:H7 to acetic acid (AA) or malic acid (MA) by adding osmolytes, such as glycerol, glycine, glucose, and fructose, in Luria-Bertani broth without NaCl (LB) or phosphate buffer (PB) stored at 25 °C. In LB, a significantly (p < 0.
View Article and Find Full Text PDF: Direct-acting antiviral (DAA) therapy can cure chronic hepatitis C (CHC), and daclatasvir (DCV)/asunaprevir (ASV) was the first interferon-free DAA therapy introduced in Korea. Patients who achieve sustained virologic response (SVR) after DAA treatment are expected to have good prognoses. Therefore, in this study, we aimed to investigate the prognosis of these patients.
View Article and Find Full Text PDFUnlabelled: This study was conducted to develop a selective medium for enumerating LAB and Bifidobacteria in food samples. Thirteen media were evaluated to determine their suitability for selectively enumerating LAB (17 bacilli and 7 cocci) and Bifidobacteria (12 strains) under aerobic and anaerobic conditions. When BL, BCP, and mMRS were supplemented with propionic acid (5 ml/l) and adjusted to pH 5, the growth of all indicator microorganisms was inhibited; however, these media also inhibited the growth of certain LAB and Bifidobacterial strains.
View Article and Find Full Text PDFThis study sought to І)investigate the effect of varying concentrations of salt on the survival of Gram-negative bacteria in artificial seawater (ASW; pH 6) stored at 4 °C, ІІ)examine the culturable populations of Vibrio vulnificus persisting in ASW with or without 1000 U/mg catalase or 0.1% sodium pyruvate (pH 6) under aerobic and oxygen-restricted environments at 4 °C, and ІІІ)determine whether double layer agar plate (DLAP) methods can improve the culturability of V. vulnificus and Vibrio parahaemolyticus in ASW (pH 6) stored at 4 °C.
View Article and Find Full Text PDFUnlabelled: This study aimed to characterize the bacterial community of commercial potting soils with or without inoculation at 5-35 °C using 16S metagenomic sequencing and evaluate the effect of natural amendments on the reduction . in non-sterile potting soils. An increase in the expected operational taxonomic units of each sample with or without was proportional to the increasing storage temperatures after 5 days.
View Article and Find Full Text PDFZbtb7c is a proto-oncoprotein that controls the cell cycle and glucose, glutamate, and lipid metabolism. Zbtb7c expression is increased in the liver and white adipose tissues of aging or high-fat diet-fed mice. Knockout or knockdown of Zbtb7c gene expression inhibits the adipocyte differentiation of 3T3-L1 cells and decreases adipose tissue mass in aging mice.
View Article and Find Full Text PDFUnlabelled: This study was undertaken to develop enhanced selective media for detection of in oysters. Primarily, tryptic soy agar (TSA) was supplemented with 4.5-5% NaCl, 0.
View Article and Find Full Text PDFThis study was conducted to examine the effect of formulated resuscitation-promoting broths on the revival of viable but nonculturable induced by cold and starvation stresses. was incubated in artificial sea water at 4 °C for more than 8 months until this bacterium became undetectable, while retaining its intact cell count of more than 10 CFU/field over time. On day 250, .
View Article and Find Full Text PDFIn this study, a total of 195 samples including fresh produce and farming environments was used to perform the microbial risk assessment. Levels of total aerobic bacteria ranged from 2.77 to 5.
View Article and Find Full Text PDFCrit Rev Food Sci Nutr
April 2020
has been consistently found to be involved in the food-borne disease outbreaks every year. Particularly, can be induced into a viable but nonculturable (VBNC) state under cold-starvation conditions. In this physiological state, losses its colony-forming ability and shows reduced metabolic activities.
View Article and Find Full Text PDFA yellow-pigmented novel bacterial strain, MAH-12, was isolated from a soil sample of Korean radish garden and was characterized using a polyphasic approach. Cells were Gram-staining negative, non-motile and rod-shaped. The strain was aerobic, catalase positive, optimum growth temperature and pH were 28-30 °C and 6.
View Article and Find Full Text PDFGiven that it should be aware of the nutritional benefits, resulting from the consumption of fresh fruits and vegetables consumed raw and/or minimally processed, comparing the efficacy of different individual sanitizing methods against the major food-borne pathogens localized in fresh commodities is of much importance; these products are easily vulnerable to the microbial contamination. In this review, the current propensity of alternative sanitizing methods was introduced, as well as principal elements for deteriorating the cleaning effects were also discussed. Chlorine-based-sanitizers exhibited the microbial reduction of <1.
View Article and Find Full Text PDFThere has been limited information available on the behavior of and as a function of higher levels of NaCl in combination with acidic pH. In the present study, bacterial suspensions were transferred into artificial seawater (pH 4-7) microcosms containing 0.75% NaCl and supplemented with 5, 10, and 30% NaCl, respectively.
View Article and Find Full Text PDFThe effectiveness of sanitizing treatments was investigated on reducing pathogens inoculated in whole or cut fresh vegetables, including Brussels sprouts, carrots, cherry tomatoes, paprika, and lettuce. These products were inoculated with O157:H7, serovar Typhimurium, and and then treated with chlorine and alcohol sanitizers, followed by the subsequent washing procedure in sterile distilled water at 25°C for 5min. Alcohol sanitizer was the most effective in inhibiting O157:H7, S.
View Article and Find Full Text PDFFood Sci Biotechnol
December 2016
In Southeast Asian countries, including Korea, China, and Japan, the considerable amounts of raw fish have been annually consumed in the manner of live fish fillets without minimally thermal processing, increasing the risks of causing food-borne diseases. This study investigated the occurrence of total aerobic bacteria (TAB), coliform, , serovar spp., , and in jacopevers and plaices.
View Article and Find Full Text PDFCXXC5 is a member of a small subset of proteins containing CXXC-type zinc-finger domain. Here, we show that CXXC5 is a transcription factor activating Flk-1, a receptor for vascular endothelial growth factor. CXXC5 and Flk-1 were accumulated in nucli and membrane of mouse embryonic stem cells (mESCs), respectively, during their endothelial differentiation.
View Article and Find Full Text PDF